The distribution of intestinal flora after hematopoietic stem cell transplantation in children

作者全名："Tong, Lin; Meng, Yan; Zhang, Luying; Yu, Jie; Dou, Ying"

作者地址："[Tong, Lin; Meng, Yan; Zhang, Luying; Yu, Jie; Dou, Ying] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Child Infect & Immun, China Int Sci & Technol Cooperat Base Child Dev &, 136,Zhongshan 2nd Rd, Chongqing 400014, Peoples R China"

通信作者："Dou, Y (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Chongqing Key Lab Child Infect & Immun, China Int Sci & Technol Cooperat Base Child Dev &, 136,Zhongshan 2nd Rd, Chongqing 400014, Peoples R China."

来源：PEDIATRIC TRANSPLANTATION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001130414400001

JCR分区：Q3

影响因子：1.2

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：gastrointestinal graft versus host disease; hematopoietic stem cell transplantation; intestinal flora

摘要："BackgroundThis prospective study aimed to comprehensively understand the changes in intestinal flora at different stages after hematopoietic stem cell transplantation (HSCT) in pediatric patients and to analyze the effect of intestinal flora on acute graft versus host disease (aGVHD), especially on gastrointestinal graft versus host disease (GI GVHD).MethodsA total of 32 children with primary diseases of primary immunodeficiency disease (PID) and thalassemia were included. 16S sequencing was used to characterize the microbiota layout at three time points peri-transplant including pre-transplant, Day +3, and Day +30.ResultsBy comparing the intestinal flora of children with GI GVHD and those without GI GVHD, it suggests that in children with GI GVHD, the distribution of intestinal flora after transplantation was more variable and more chaotic (chao1 index, Friedman test, p = .029). Besides, Veillonella and Ruminococcaceae were more abundant before transplantation, Bifidobacteriaceae and Bacillales were more abundant after transplantation. Comparing children with PID and thalassemia, it was found that the destruction of gut microbiota diversity was more significant in children with thalassemia after transplantation. The comparison of children with 0-I degrees aGVHD and II-III degrees aGVHD indicates that children with II-III degrees aGVHD had more Bilophila before transplantation than children with 0-I degrees aGVHD. Additionally, exploratory analyses to evaluate correlations between clinical characteristics (medications, immune cell recovery, etc.) and microbiome features were also performed.ConclusionsThis study has synthetically shown the distribution of intestinal flora after allo-HSCT, and some characteristic bacteria at different stages that may serve as potential biomarkers were screened out additionally, perhaps providing clues for the prevention and treatment of the disease."

基金机构：The National Natural Science Foundation of China Youth Science Fund Project

基金资助正文：No Statement Availabler No Statement Availabler No Statement Available