Identification of Adenovirus E1B-55K Interaction Partners through a Common Binding Motif

作者全名："Hagkarim, Nafiseh Chalabi; Ip, Wing-Hang; Bertzbach, Luca D.; Abualfaraj, Tareq; Dobner, Thomas; Molloy, David P.; Stewart, Grant S.; Grand, Roger J."

作者地址："[Hagkarim, Nafiseh Chalabi; Stewart, Grant S.; Grand, Roger J.] Univ Birmingham, Med Sch, Inst Canc & Genom Sci, Birmingham B15 2TT, England; [Ip, Wing-Hang; Bertzbach, Luca D.; Dobner, Thomas] Leibniz Inst Virol, Dept Viral Transformat, D-20251 Hamburg, Germany; [Abualfaraj, Tareq] Taibah Univ, Dept Med Microbiol & Immunol, POB 344, Madinah 41477, Saudi Arabia; [Molloy, David P.] Chongqing Med Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Chongqing 400016, Peoples R China; Inst Canc Res, London SW7 3RP, England"

通信作者："Grand, RJ (通讯作者)，Univ Birmingham, Med Sch, Inst Canc & Genom Sci, Birmingham B15 2TT, England."

来源：VIRUSES-BASEL

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001131170600001

JCR分区：Q2

影响因子：3.8

年份：2023

卷号：15

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：adenovirus; cullin; early region 1B; MRE11; p53; p53 binding motif; PR619; USP

摘要："The adenovirus C5 E1B-55K protein is crucial for viral replication and is expressed early during infection. It can interact with E4orf6 to form a complex that functions as a ubiquitin E3 ligase. This complex targets specific cellular proteins and marks them for ubiquitination and, predominantly, subsequent proteasomal degradation. E1B-55K interacts with various proteins, with p53 being the most extensively studied, although identifying binding sites has been challenging. To explain the diverse range of proteins associated with E1B-55K, we hypothesized that other binding partners might recognize the simple p53 binding motif (xWxxxPx). In silico analyses showed that many known E1B-55K binding proteins possess this amino acid sequence; therefore, we investigated whether other xWxxxPx-containing proteins also bind to E1B-55K. Our findings revealed that many cellular proteins, including ATR, CHK1, USP9, and USP34, co-immunoprecipitate with E1B-55K. During adenovirus infection, several well-characterized E1B-55K binding proteins and newly identified interactors, including CSB, CHK1, and USP9, are degraded in a cullin-dependent manner. Notably, certain binding proteins, such as ATR and USP34, remain undegraded during infection. Structural predictions indicate no conservation of structure around the proposed binding motif, suggesting that the interaction relies on the correct arrangement of tryptophan and proline residues."

基金机构：CR-UK

基金资助正文：No Statement Available