SPAG6 regulates cell proliferation and apoptosis via TGF-β/Smad signal pathway in adult B-cell acute lymphoblastic leukemia

作者全名："Zhao, Beibei; Yin, Jiaxiu; Ding, Li; Luo, Jie; Luo, Jing; Mu, Jiao; Pan, Shirui; Du, Juan; Zhong, Yirui; Zhang, Linyi; Liu, Lin"

作者地址："[Zhao, Beibei; Yin, Jiaxiu; Ding, Li; Luo, Jie; Luo, Jing; Mu, Jiao; Pan, Shirui; Zhong, Yirui; Zhang, Linyi; Liu, Lin] Chongqing Med Univ, Dept Hematol, Med Coll Rd, Chongqing, Peoples R China; [Zhao, Beibei; Yin, Jiaxiu; Ding, Li; Luo, Jie; Luo, Jing; Mu, Jiao; Pan, Shirui] Chongqing Med Univ, Affiliated Hosp 1, Lab Res Ctr, Chongqing 400042, Peoples R China; [Ding, Li; Du, Juan] Southwest Med Univ, Dept Hematol, Affiliated Hosp, Luzhou 646000, Sichuan, Peoples R China"

通信作者："Liu, L (通讯作者)，Chongqing Med Univ, Dept Hematol, Med Coll Rd, Chongqing, Peoples R China."

来源：INTERNATIONAL JOURNAL OF HEMATOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001131538900001

JCR分区：Q3

影响因子：1.7

年份：2023

卷号：　

期号：　

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文献类型：Article; Early Access

关键词：Adult B-cell acute lymphoblastic leukemia; SPAG6; TGF-beta/Smad; Proliferation; Apoptosis

摘要："Adult B-cell acute lymphoblastic leukemia (B-ALL) prognosis remains unsatisfactory, and searching for new therapeutic targets is crucial for improving patient prognosis. Sperm-associated antigen 6 (SPAG6), a member of the cancer-testis antigen family, plays an important role in tumors, especially hematologic tumors; however, it is unknown whether SPAG6 plays a role in adult B-ALL. In this study, we demonstrated for the first time that SPAG6 expression was up-regulated in the bone marrow of adult B-ALL patients compared to healthy donors, and expression was significantly reduced in patients who achieved complete remission (CR) after treatment. In addition, patients with high SPAG6 expression were older (>= 35 years; P = 0.015), had elevated white blood cell counts (WBC > 30 x 10(9)/L; P = 0.021), and a low rate of CR (P = 0.036). We explored the SPAG6 effect on cell function by lentiviral transfection of adult B-ALL cell lines BALL-1 and NALM-6, and discovered that knocking down SPAG6 significantly inhibited cell proliferation and promoted apoptosis. We identified that SPAG6 knockdown might regulate cell proliferation and apoptosis via the transforming growth factor-beta (TGF-beta)/Smad signaling pathway."

基金机构：National Natural Science Foundation of China; Experimental Research Center of the First Affiliated Hospital of Chongqing Medical University

基金资助正文："The authors would like to thank the Experimental Research Center of the First Affiliated Hospital of Chongqing Medical University (Chongqing, China) for their technical support. The authors would also like to thank the Molecular Testing Center of the First Affiliated Hospital of Chongqing Medical University for providing frozen bone marrow specimens from patients and healthy donors."