Metabolic Patterns of High-Invasive and Low-Invasive Oral Squamous Cell Carcinoma Cells Using Quantitative Metabolomics and <SUP>13</SUP>C-Glucose Tracing

作者全名："Jiang, Wenrong; Zhang, Ting; Zhang, Hua; Han, Tingli; Ji, Ping; Ou, Zhanpeng"

作者地址："[Jiang, Wenrong; Zhang, Ting; Ji, Ping; Ou, Zhanpeng] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Jiang, Wenrong; Zhang, Ting; Ji, Ping; Ou, Zhanpeng] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401147, Peoples R China; [Jiang, Wenrong; Zhang, Ting; Ji, Ping; Ou, Zhanpeng] Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China; [Zhang, Hua; Han, Tingli] Chongqing Med Univ, Int Collaborat Joint Lab Reprod & Dev, Minist Educ China, Chongqing 400016, Peoples R China; [Zhang, Hua] Chongqing Med Univ, State Key Lab Maternal & Fetal Med Chongqing Munic, Chongqing 400016, Peoples R China; [Zhang, Hua] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China; [Zhang, Hua] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing 400016, Peoples R China; [Han, Tingli] Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Chongqing, Peoples R China"

通信作者："Ji, P; Ou, ZP (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China.; Ji, P; Ou, ZP (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401147, Peoples R China.; Ji, P; Ou, ZP (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing 401147, Peoples R China."

来源：BIOMOLECULES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001132108600001

JCR分区：Q1

影响因子：4.8

年份：2023

卷号：13

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：quantitative metabolomics; stable isotope tracing; oral squamous cell carcinoma cells; metastasis

摘要："Most current metabolomics studies of oral squamous cell carcinoma (OSCC) are mainly focused on identifying potential biomarkers for early screening and diagnosis, while few studies have investigated the metabolic profiles promoting metastasis. In this study, we aimed to explore the altered metabolic pathways associated with metastasis of OSCC. Here, we identified four OSCC cell models (CAL27, HN6, HSC-3, SAS) that possess different invasive heterogeneity via the transwell invasion assay and divided them into high-invasive (HN6, SAS) and low-invasive (CAL27, HSC-3) cells. Quantitative analysis and stable isotope tracing using [U-C-13(6)] glucose were performed to detect the altered metabolites in high-invasive OSCC cells, low-invasive OSCC cells and normal human oral keratinocytes (HOK). The metabolic changes in the high-invasive and low-invasive cells included elevated glycolysis, increased fatty acid metabolism and an impaired TCA cycle compared with HOK. Moreover, pathway analysis demonstrated significant differences in fatty acid biosynthesis; arachidonic acid (AA) metabolism; and glycine, serine and threonine metabolism between the high-invasive and low-invasive cells. Furthermore, the high-invasive cells displayed a significant increase in the percentages of C-13-glycine, C-13-palmitate, C-13-stearic acid, C-13-oleic acid, C-13-AA and estimated FADS1/2 activities compared with the low-invasive cells. Overall, this exploratory study suggested that the metabolic differences related to the metastatic phenotypes of OSCC cells were concentrated in glycine metabolism, de novo fatty acid synthesis and polyunsaturated fatty acid (PUFA) metabolism, providing a comprehensive understanding of the metabolic alterations and a basis for studying related molecular mechanisms in metastatic OSCC cells."

基金机构：The National Natural Science Foundation of China

基金资助正文："The authors acknowledge other participants in our laboratory for their active help in this study. The authors appreciate all the reviewers for their useful comments, which also helped to improve the quality of the manuscript."