PdPtB Electrochemiluminescence Nanoenhancer and SiC@Au-PEDOT Nanowires-Based Detection of β-Amyloid Oligomers in Alzheimer's Disease

作者全名："Peng, Lingshuang; Min, Weiziyang; Chen, Rui; Zhang, Lu; Shen, Bo; Xu, Wenchun; Liu, Changjin"

作者地址："[Peng, Lingshuang; Min, Weiziyang; Chen, Rui; Zhang, Lu; Shen, Bo; Xu, Wenchun; Liu, Changjin] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China; [Min, Weiziyang] Nanchang Univ, Queen Mary Sch, Nanchang 330036, Peoples R China"

通信作者："Xu, WC; Liu, CJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China."

来源：ACS APPLIED MATERIALS & INTERFACES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001133440600001

JCR分区：Q1

影响因子：8.3

年份：2023

卷号：15

期号：51

开始页：59189

结束页：59198

文献类型：Article

关键词：beta-amyloid oligomers; Alzheimer's disease; electrochemiluminescence immunosensor; PdPtB nanoenhancer; SiC@Au-PEDOT nanowires; reactive oxygen species

摘要："beta-Amyloid oligomers (A beta Os) are promising biomarkers for the diagnosis of Alzheimer's disease (AD). The present research introduces a novel electrochemiluminescence (ECL) immunosensor based on PdPtB nanoenhancer and SiC@Au-PEDOT nanowires (NWs) for the specific and ultrasensitive detection of A beta Os. The PdPtB nanoenhancer exhibited excellent oxidase-like catalytic activity with in situ generation of reactive oxygen species (ROS) to enhance luminol ECL in neutral media. In addition, SiC@Au-PEDOT NWs were utilized as a biocompatible and conductive substrate for the modification of the glassy carbon electrode (GCE). With this design, the ECL immunosensor showed outstanding A beta Os analytical performance without exogenous coreactant. The ECL immunosensor demonstrated a favorable linear range of 20 pM to 20 nM and a detection limit of 10 pM under optimized conditions with potential straightforward clinical application. In general, the developed ECL immunosensor provides a promising strategy for the early diagnosis of AD."

基金机构："China Postdoctoral Science Foundation [82202642]; National Natural Science Foundation of China [2023MD734121, 2022M710558]; China Postdoctoral Science Foundation [CQBX202218]; Chongqing Postdoctoral Innovation Talent Support Program [2021XM2036]; Special foundation of Chongqing Postdoctoral Research Programme [CSTB2022NSCQ-BHX0689]; Chongqing Postdoctoral Science Foundation"

基金资助正文："This work was funded by the National Natural Science Foundation of China (82202642), China Postdoctoral Science Foundation (2023MD734121, 2022M710558), Chongqing Postdoctoral Innovation Talent Support Program (CQBX202218), Special foundation of Chongqing Postdoctoral Research Programme (2021XM2036), Chongqing Postdoctoral Science Foundation (CSTB2022NSCQ-BHX0689)."