A Low-cost Creatinine Biosensor by Differential Optical Signal Readout for the Whole Blood Analysis

作者全名："Cheng, Jie; Guo, Jiuchuan; Guo, Jinhong"

作者地址："[Cheng, Jie] Univ Elect Sci & Technol China, Sch Informat & Commun Engn, Chengdu 611731, Peoples R China; [Guo, Jiuchuan] Univ Elect Sci & Technol China, Sch Automat Engn, Chengdu 611731, Peoples R China; [Guo, Jinhong] Univ Elect Sci & Technol China, Chengdu 611731, Peoples R China; [Guo, Jinhong] Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai, Peoples R China; [Guo, Jinhong] Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China"

通信作者："Guo, JH (通讯作者)，Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai, Peoples R China.; Guo, JH (通讯作者)，Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China."

来源："2023 45TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE & BIOLOGY SOCIETY, EMBC"

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WOS号：WOS:001133788305075

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年份：2023

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文献类型：Proceedings Paper

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摘要："This study developed a low-cost paper-based biosensor for point-of-care (POC) detection of blood creatinine by using differential optical signal readout. Dual-channel photochemical paper-based test strips were fabricated with stackable multilayer films containing pre-immobilized enzymes and reagents for the identification and conversion of creatinine and creatine. Enzyme-linked reactions generated hydrogen peroxide (H2O2), which formed a blue oxidized condensate with aniline derivatives. The color depth was quantified via the differential optical signal of the two channels and positively correlated with the concentration of the analyte. This method was first proposed to address the issue of endogenous interferences in the enzymatic assay of creatinine, greatly improving the detection accuracy. The proposed biosensor was calibrated with spiked blood samples, and achieved a wide detection range of 31-1483 mu mol/L, showing superior detection performance to general enzymatic methods, especially in the low concentration range. Creatine interference testing demonstrated that the biosensor could resist the interference of <= 300 mu mol/L endogenous creatine. It is believed that the proposed optical differential biosensor for blood creatinine could enable to pave the way for a daily monitoring system for renal diseases."

基金机构：National Natural Science Foundation of China [62122017]

基金资助正文：The authors thank the financial support from National Natural Science Foundation of China (62122017).