Structure and Separation Analysis of Human Milk Oligosaccharides

作者全名："Ma, Xin-Yue; Huang, Chun-Cui; Zhao, Yao; Li, Yan"

作者地址："[Ma, Xin-Yue; Huang, Chun-Cui; Li, Yan] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Ma, Xin-Yue; Li, Yan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhao, Yao] Chongqing Med Univ, Childrens Hosp, Chongqing 400015, Peoples R China"

通信作者："Li, Y (通讯作者)，Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China.; Li, Y (通讯作者)，Univ Chinese Acad Sci, Beijing 100049, Peoples R China."

来源：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001134817800007

JCR分区：Q4

影响因子：0.2

年份：2023

卷号：50

期号：12

开始页：2869

结束页：2878

文献类型：Article

关键词：human milk oligosaccharides; isolation; structure elucidation; mass spectrometry

摘要："Human milk oligosaccharides (HMOs) present in breast milk are a family of highly complex oligosaccharides that play a positive role in the intestinal flora, immune barrier, and brain development of infants. Due to the complex matrix in breast milk, the variety of oligosaccharides, the span of abundance, and the presence of many isomers, the detection of breast milk is faced with many challenges. A variety of techniques have been used for the analysis of human milk oligosaccharides and over 200 HMOs have been identified. Liquid chromatography and capillary electrophoresis have been effective in separating human milk oligosaccharides. Nuclear magnetic resonance spectroscopy, mass spectrometry and infrared multiphoton dissociation spectroscopy have promoted the comprehensive structural resolution of HMOs. This paper reviews the various techniques used to achieve high sensitivity and specificity for the analysis of HMOs and compares the advantages and disadvantages of the different techniques. It also focuses on the breakthroughs in mass spectrometry and the combination of different techniques to facilitate the analysis and determination of HMOs, providing a comprehensive technical support to explore the structure-function relationship of oligosaccharides and to gain a deeper understanding of the biological functions of HMOs."

基金机构："National Natural Science Foundation of China [62072435, 82130055]; National Key R&D Program of China [2022YFC3400801]; Chinese Academy of Sciences [KFJ-BRP-017-76]"

基金资助正文："This work was supported by grants from The National Natural Science Foundation of China (62072435, 82130055), The National Key R & D Program of China (2022YFC3400801), and Biological Resources Program, Chinese Academy of Sciences (KFJ-BRP-017-76)."