Development of a ferroptosis-based molecular markers for predicting RFS in prostate cancer patients

作者全名："Chen, Jinquan; Zhang, Longbin; Luo, Yiling; Tan, Chao; Hu, Huang; Jiang, Yuling; Xi, Na; Zeng, Qinghai; Peng, H."

作者地址："[Chen, Jinquan; Zhang, Longbin; Luo, Yiling; Tan, Chao; Hu, Huang; Jiang, Yuling; Xi, Na; Zeng, Qinghai] Tongnan Dist Peoples Hosp, Dept Ophthalmol, Chongqing, Peoples R China; [Peng, H.] Chongqing Med Univ, Affiliated Hosp 1, Dept Ophthalmol, Chongqing, Peoples R China"

通信作者："Peng, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Ophthalmol, Chongqing, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:001136268000008

JCR分区：Q1

影响因子：3.8

年份：2023

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："The goal of this study was to develop a ferroptosis-based molecular signature that can predict recurrence-free survival (RFS) in patients with prostate cancer (PCa). In this study, we obtained ferroptosis-related genes (FRGs) in FerrDb database and clinical transcriptome data in TCGA database and GEO database. Consensus cluster analysis was used to identify three molecular markers of ferroptosis in PCa with differential expression of 40 FRGs, including PD-L1 expression levels. We conducted a new ferroptosis-related signature for PCa RFS using four FRGs identified through univariate and multivariate Cox regression analyses. The signature was validated in the training, testing, and validation cohorts, and it demonstrated remarkable results in the area under the time-dependent receiver operating characteristic (ROC) curve of 0.757, 0.715, and 0.732, respectively. Additionally, we observed that younger patients, those with stage T III and stage T IV, stage N0, cluster 1, and cluster 2 PCa were more accurately predicted by the signature as independent predictors of RFS. DU-145 and RWPE-1 cells were successfully analyzed by qRT-PCR and Western blot for ASNS, GPT2, RRM2, and NFE2L2. In summary, we developed a novel ferroptosis-based signature for RFS in PC, utilizing four FRGs identified through univariate and multivariate Cox regression analyses. This signature was rigorously validated across training, testing, and validation cohorts, demonstrating exceptional performance as evidenced by its ROC curves. Notably, our findings indicate that this signature is particularly effective as an independent predictor of RFS in younger patients or those with stage T III and T IV, stage N0, and in clusters 1 and 2. Finally, we confirmed the expression of these four FRGs in DU-145 and RWPE-1 cell lines."

基金机构：National Natural Science Foundation of China

基金资助正文："The authors are grateful to Professor PH for providing the platform, financial support and writing guidance for this experiment."