Chronic restraint stress promotes oral squamous cell carcinoma development by inhibiting ALDH3A1 via stress response hormone

作者全名："Luo, Shihong; Long, Huiqing; Lou, Fangzhi; Liu, Yiyun; Wang, Haiyang; Pu, Juncai; Ji, Ping; Jin, Xin"

作者地址："[Luo, Shihong; Long, Huiqing; Lou, Fangzhi; Ji, Ping; Jin, Xin] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China; [Luo, Shihong; Long, Huiqing; Lou, Fangzhi; Ji, Ping; Jin, Xin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Liu, Yiyun; Wang, Haiyang; Pu, Juncai] Chongqing Med Univ, Affiliated Hosp 1, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing 400042, Peoples R China"

通信作者："Jin, X (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China.; Jin, X (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China."

来源：BMC ORAL HEALTH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001138274300002

JCR分区：Q1

影响因子：2.9

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Chronic restraint stress; Hormone; Oral squamous cell carcinoma; Mitochondrial metabolism

摘要："BackgroundChronic restraint stress (CRS) has iteratively been reported to be possibly implicated in the development of numerous cancer types. However, its role in oral squamous cell carcinoma (OSCC) has not been well elucidated. Here we intended to evaluate the role and mechanism.MethodsThe effects of CRS were investigated in xenograft models of OSCC by using transcriptome sequencing, LC-MS, ELISA and RT-PCR. Moreover, the role of CRS and ALDH3A1 on OSCC cells was researched by using Trans-well, flow cytometry, western blotting, immunofluorescence, ATP activity and OCR assay. Furthermore, immunohistochemical staining was employed to observe the cell proliferation and invasion of OSCC in xenotransplantation models.ResultsCRS promoted the progression of OSCC in xenograft models, stimulated the secretion of norepinephrine and the expression of ADRB2, but decreased the expression of ALDH3A1. Moreover, CRS changed energy metabolism and increased mitochondrial metabolism markers. However, ALDH3A1 overexpression suppressed proliferation, EMT and mitochondrial metabolism of OSCC cells.ConclusionInhibition of ALDH3A1 expression plays a pivotal role in CRS promoting tumorigenic potential of OSCC cells, and the regulatory of ALDH3A1 on mitochondrial metabolism may be involved in this process."

基金机构：National Natural Science Foundations of China

基金资助正文：None.