Intravenous Senescent Erythrocyte Vaccination Modulates Adaptive Immunity and Splenic Complement Production

作者全名："He, Yan; Cheng, Chuanxu; Liu, Yuheng; Chen, Fang-Man; Chen, Yinglu; Yang, Chao; Zhao, Zhibin; Dawulieti, Jianati; Shen, Zikun; Zhang, Yunjiao; Du, Jin-Zhi; Guan, Shan; Shao, Dan"

作者地址："[He, Yan; Cheng, Chuanxu; Chen, Fang-Man; Yang, Chao; Dawulieti, Jianati; Shao, Dan] South China Univ Technol, Sch Biomed Sci & Engn, Guangzhou 511442, Guangdong, Peoples R China; [Liu, Yuheng; Guan, Shan] Third Mil Med Univ, Natl Engn Res Ctr Immunol Prod, Chongqing 400038, Peoples R China; [Liu, Yuheng] Chongqing Med Univ, Coll Pharm, Dept Pharmacol, Chongqing 400016, Peoples R China; [Chen, Yinglu; Shen, Zikun; Zhang, Yunjiao; Du, Jin-Zhi; Shao, Dan] South China Univ Technol, Sch Med, Guangzhou 510006, Guangdong, Peoples R China; [Yang, Chao] Southern Med Univ, Orthoped Hosp Guangdong Prov, Guangdong Prov Key Lab Bone & Joint Degenerat Dis, Dept Orthoped,Acad Orthoped Guangdong Prov,Affilia, Guangzhou 510665, Peoples R China; [Zhao, Zhibin] Southern Med Univ, Guangdong Prov Peoples Hosp, Med Res Inst, Guangdong Acad Med Sci, Guangzhou 510080, Guangdong, Peoples R China; [Shao, Dan] South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China"

通信作者："Yang, C; Shao, D (通讯作者)，South China Univ Technol, Sch Biomed Sci & Engn, Guangzhou 511442, Guangdong, Peoples R China.; Guan, S (通讯作者)，Third Mil Med Univ, Natl Engn Res Ctr Immunol Prod, Chongqing 400038, Peoples R China.; Shao, D (通讯作者)，South China Univ Technol, Sch Med, Guangzhou 510006, Guangdong, Peoples R China.; Yang, C (通讯作者)，Southern Med Univ, Orthoped Hosp Guangdong Prov, Guangdong Prov Key Lab Bone & Joint Degenerat Dis, Dept Orthoped,Acad Orthoped Guangdong Prov,Affilia, Guangzhou 510665, Peoples R China.; Shao, D (通讯作者)，South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China."

来源：ACS NANO

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001139471800001

JCR分区：Q1

影响因子：15.8

年份：2023

卷号：18

期号：1

开始页：470

结束页：482

文献类型：Article

关键词：senescent erythrocyte; splenic targeting; biomimeticvaccine; complement production; immunotherapy

摘要："Targeted delivery of vaccines to the spleen remains a challenge. Inspired by the erythrophagocytotic process in the spleen, we herein report that intravenous administration of senescent erythrocyte-based vaccines profoundly alters their tropism toward splenic antigen-presenting cells (APCs) for imprinting adaptive immune responses. Compared with subcutaneous inoculation, intravenous vaccination significantly upregulated splenic complement expression in vivo and demonstrated synergistic antibody killing in vitro. Consequently, intravenous senescent erythrocyte vaccination produces potent SARS-CoV-2 antibody-neutralizing effects, with potential protective immune responses. Moreover, the proposed senescent erythrocyte can deliver antigens from resected tumors and adjuvants to splenic APCs, thereby inducing a personalized immune reaction against tumor recurrence after surgery. Hence, our findings suggest that senescent erythrocyte-based vaccines can specifically target splenic APCs and evoke adaptive immunity and complement production, broadening the tools for modulating immunity, helping to understand adaptive response mechanisms to senescent erythrocytes better, and developing improved vaccines against cancer and infectious diseases."

基金机构："Fundamental Research Funds for the Central Universities [32271388, 32101073]; National Natural Science Foundation of China [202201010634, 2021YFC2302500]; Guangzhou Basic and Applied Basic Research Foundation; Ministry of Science and Technology of China [CQYC202005027]; Chongqing Talents: Exceptional Young Talents Project [cstc2021jcyj-msxmX0136]; Natural Science Foundation of Chongqing; Fundamental Research Funds for the Central Universities"

基金资助正文："This work was supported by National Natural Science Foundation of China (Nos. 32271388 and 32101073), Guangzhou Basic and Applied Basic Research Foundation (No. 202201010634), the major project of Study on Pathogenesis and Epidemic Prevention Technology System (2021YFC2302500) by the Ministry of Science and Technology of China, the Chongqing Talents: Exceptional Young Talents Project (CQYC202005027), the Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0136) and the Fundamental Research Funds for the Central Universities."