Multilocus Distance-Regulated Sensor Array for Recognition of Polyphenols via Machine Learning and Indicator Displacement Assay

作者全名："Ni, Weiwei; Yu, Yang; Gao, Xu; Han, Yang; Zhang, Wenhui; Zhang, Zerui; Xiao, Wenqi; Hu, Qin; Zhang, Yanliang; Huang, Hui; Li, Fei; Chen, Mingqi; Han, Jinsong"

作者地址："[Ni, Weiwei; Gao, Xu; Zhang, Wenhui; Zhang, Zerui; Xiao, Wenqi; Huang, Hui; Li, Fei; Chen, Mingqi; Han, Jinsong] China Pharmaceut Univ, Coll Engn, State Key Lab Nat Med, Nanjing 211109, Peoples R China; [Yu, Yang] China Pharmaceut Univ, Sch Tradit Chinese Pharm, Nanjing 211109, Peoples R China; [Han, Yang] China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing 211109, Peoples R China; [Hu, Qin] Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Zhang, Yanliang] Nanjing Univ Chinese Med, Nanjing Res Ctr Infect Dis Integrated Tradit Chine, Nanjing Hosp Chinese Med, Nanjing 211109, Peoples R China"

通信作者："Li, F; Chen, MQ; Han, JS (通讯作者)，China Pharmaceut Univ, Coll Engn, State Key Lab Nat Med, Nanjing 211109, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:001139533600001

JCR分区：Q1

影响因子：6.7

年份：2023

卷号：96

期号：1

开始页：301

结束页：308

文献类型：Article

关键词：　

摘要："Developing new strategies to construct sensor arrays that can effectively distinguish multiple natural components with similar structures in mixtures is an exceptionally challenging task. Here, we propose a new multilocus distance-modulated indicator displacement assay (IDA) strategy for constructing a sensor array, incorporating machine learning optimization to identify polyphenols. An 8-element array, comprising two fluorophores and their six dynamic covalent complexes (C1-C6) formed by pairing two fluorophores with three distinct distance-regulated quenchers, has been constructed. Polyphenols with diverse spatial arrangements and combinatorial forms compete with the fluorophores by forming pseudocycles with quenchers within the complexes, leading to varying degrees of fluorescence recovery. The array accurately and effectively distinguished four tea polyphenols and 16 tea varieties, thereby demonstrating the broad applicability of the multilocus distance-modulated IDA array in detecting polyhydroxy foods and natural medicines."

基金机构："National Natural Science Foundation of China [52003298, 32272415, 82072017]; National Natural Science Foundation of China [BK20200578]; Natural Science Foundation of Jiangsu Province [CPUQNJC22_04]; Funding of Double First-Rate Discipline Innovation Team"

基金资助正文："The authors are grateful for financial support from the National Natural Science Foundation of China (52003298, 32272415, and 82072017), the Natural Science Foundation of Jiangsu Province (BK20200578), and the Funding of Double First-Rate Discipline Innovation Team (CPUQNJC22_04). The authors thank Dr. Hui-Min Xu from the Public Laboratory Platform at China Pharmaceutical University for assistance with NMR techniques."