"S ilencing of FAM111B inhibited proliferation, migration and invasion of hepatoma cells through activating p53 pathway"

作者全名："Li, Feng; He, Hong-ye; Fan, Zhi-hao; Li, Chun-ming; Gong, Yi; Wang, Xiao-jun; Xiong, Hao-jun; Xie, Chuan-ming; Bie, Ping"

作者地址："[Li, Feng; Fan, Zhi-hao; Bie, Ping] Chongqing Med Univ, Affiliated Hosp 3, Dept Hepatobiliary & Pancreat Surg, Chongqing 401120, Peoples R China; [He, Hong-ye] Chongqing Med Univ, Inst Ultrasound Imaging, Chongqing Key Lab Ultrasound Mol Imaging, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [He, Hong-ye] Chongqing Med Univ, Affiliated Hosp 2, Dept Ultrasound, Chongqing Key Lab Ultrasound Mol Imaging, Chongqing 400010, Peoples R China; [Li, Chun-ming] Chongqing Med Univ, Affiliated Hosp 2, Dept Hepatobiliary Surg, Chongqing 400010, Peoples R China; [Gong, Yi; Wang, Xiao-jun] Army Med Univ, Southwest Hosp, Dept Hepatobiliary & Pancreat Surg, Chongqing 400038, Peoples R China; [Xiong, Hao-jun; Xie, Chuan-ming] Army Med Univ, Southwest Hosp, Inst Hepatobiliary Surg, Key Lab Hepatobiliary & Pancreat Surg, Chongqing 400038, Peoples R China; [Xiong, Hao-jun; Xie, Chuan-ming; Bie, Ping] Chongqing Med Univ, Affiliated Hosp 3, Dept Hepatobiliary & Pancreat Surg, 1 Shuanghu Branch Rd,Huixing St, Chongqing, Peoples R China"

通信作者："Xiong, HJ; Xie, CM; Bie, P (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Hepatobiliary & Pancreat Surg, 1 Shuanghu Branch Rd,Huixing St, Chongqing, Peoples R China."

来源：DIGESTIVE AND LIVER DISEASE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001141475800001

JCR分区：Q1

影响因子：4

年份：2023

卷号：55

期号：12

开始页：1679

结束页：1689

文献类型：Article; Early Access

关键词：FAM111B; Migration; Proliferation; p53 pathway

摘要："Background: The function of Family with sequence similarity 111 member B (FAM111B) has been reported in multiple malignancies, but its involvement in occurrence and development of hepatocellular carcinoma (HCC) is still unclear.Purpose: To investigate the role of FAM111B in HCC and explore the potential molecular mechanism.Methods: We examined the mRNA level of FAM111B via qPCR and protein level via immunohistochem-istry in human HCC tissues. siRNA was used to construct a FAM111B-knockdown model in HCC cell lines. CCK-8, colony formation, transwell, and wound healing assays were performed to investigate the effect of FAM111B on proliferation, migration and invasion of HCC cell. Gene Set Enrichment Analysis, western blotting, and flow cytometry were carried out to find the related molecular mechanism.Results: Human HCC tumor tissues exhibited higher expression of FAM111B, and high FAM111B expres-sion was associated with poor prognosis. Vitro assays demonstrated that knockdown of FAM111B greatly repressed proliferation, migration and invasion of HCC cells. Furthermore, silencing of FAM111B signif-icantly resulted in cell cycle arrest at G0/G1 and downregulation of epithelial-mesenchymal transition (EMT)-related proteins MMP7 and MMP9 via activation of p53 pathway.Conclusion: FAM111B played an essential role in promoting HCC development by regulation of p53 path-way.(c) 2023 Published by Elsevier Ltd on behalf of Editrice Gastroenterologica Italiana S.r.l."

基金机构：National Natural Science Foun-dation of China [31900449]

基金资助正文：<BOLD>Funding</BOLD> This project is supported by the National Natural Science Foun-dation of China (NO. 31900449) .