Low-level laser therapy alleviates periodontal age-related inflammation in diabetic mice via the GLUT1/mTOR pathway

作者全名："Cui, Aimin; Sun, Yuezhang; Zhu, Kangjian; Zou, Haonan; Yue, Ziqi; Ding, Yi; Song, Xiuxiu; Chen, Jiao; Ji, Ning; Wang, Qi"

作者地址："[Cui, Aimin; Sun, Yuezhang; Zhu, Kangjian; Zou, Haonan; Yue, Ziqi; Ding, Yi; Song, Xiuxiu; Chen, Jiao; Ji, Ning; Wang, Qi] Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China; [Cui, Aimin; Sun, Yuezhang; Zhu, Kangjian; Zou, Haonan; Yue, Ziqi; Ding, Yi; Song, Xiuxiu; Chen, Jiao; Ji, Ning; Wang, Qi] Sichuan Univ, Natl Ctr Stomatol, Chengdu 610041, Sichuan, Peoples R China; [Cui, Aimin; Sun, Yuezhang; Zhu, Kangjian; Zou, Haonan; Yue, Ziqi; Ding, Yi; Song, Xiuxiu; Chen, Jiao; Ji, Ning; Wang, Qi] Sichuan Univ, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Sichuan, Peoples R China; [Cui, Aimin; Sun, Yuezhang; Zhu, Kangjian; Zou, Haonan; Yue, Ziqi; Ding, Yi; Song, Xiuxiu; Chen, Jiao; Ji, Ning; Wang, Qi] Sichuan Univ, West China Hosp Stomatol, Chengdu 610041, Sichuan, Peoples R China; [Cui, Aimin; Sun, Yuezhang; Zhu, Kangjian; Zou, Haonan; Yue, Ziqi; Wang, Qi] Sichuan Univ, West China Hosp Stomatol, Dept Prosthodont, Chengdu 610041, Sichuan, Peoples R China; [Ding, Yi] Sichuan Univ, West China Hosp Stomatol, Dept Periodontol, Chengdu 610041, Sichuan, Peoples R China; [Song, Xiuxiu] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China"

通信作者："Wang, Q (通讯作者)，Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China.; Wang, Q (通讯作者)，Sichuan Univ, Natl Ctr Stomatol, Chengdu 610041, Sichuan, Peoples R China.; Wang, Q (通讯作者)，Sichuan Univ, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Sichuan, Peoples R China.; Wang, Q (通讯作者)，Sichuan Univ, West China Hosp Stomatol, Chengdu 610041, Sichuan, Peoples R China.; Wang, Q (通讯作者)，Sichuan Univ, West China Hosp Stomatol, Dept Prosthodont, Chengdu 610041, Sichuan, Peoples R China."

来源：LASERS IN MEDICAL SCIENCE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001143999700003

JCR分区：Q2

影响因子：2.1

年份：2024

卷号：39

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Photobiomodulation therapy; Inflammatory senescence; Hyperglycemia; Periodontal disease; Senescence-associated secretory phenotype; Cytochrome c oxidase

摘要："Diabetes mellitus (DM) is a chronic age-related disease that was recently found as a secondary aging pattern regulated by the senescence associated secretory phenotype (SASP). The purpose of this study is to detect the potential efficacy and the specific mechanisms of low-level laser therapy (LLLT) healing of age-related inflammation (known as inflammaging) in diabetic periodontitis. Diabetic periodontitis (DP) mice were established by intraperitoneal streptozotocin (STZ) injection and oral P. gingivalis inoculation. Low-level laser irradiation (810 nm, 0.1 W, 398 mW/cm2, 4 J/cm2, 10 s) was applied locally around the periodontal lesions every 3 days for 2 consecutive weeks. Micro-CT and hematoxylin-eosin (HE) stain was analyzed for periodontal soft tissue and alveolar bone. Western blots, immunohistochemistry, and immunofluorescence staining were used to evaluate the protein expression changes on SASP and GLUT1/mTOR pathway. The expression of aging-related factors and SASP including tumor necrosis factor-alpha, interleukin (IL)-1 beta, and IL-6 were reduced in periodontal tissue of diabetic mice. The inhibitory effect of LLLT on GLUT1/mTOR pathway was observed by detecting the related factors mTOR, p-mTOR, GLUT1, and PKM2. COX, an intracytoplasmic photoreceptor, is a key component of the anti-inflammatory effects of LLLT. After LLLT treatment a significant increase in COX was observed in macrophages in the periodontal lesion. Our findings suggest that LLLT may regulate chronic low-grade inflammation by modulating the GLUT1/mTOR senescence-related pathway, thereby offering a potential treatment for diabetic periodontal diseases."

基金机构：National Natural Science Foundation of China

基金资助正文：No Statement Available