A turn-on fluorescent nanosensor for H₂S detection and imaging in inflammatory cells and mice

作者全名："Liu, Xiang; Lei, Haibo; Hu, Yixiang; Fan, Xinyao; Zhang, Yazhen; Xie, Liyun; Huang, Jianji; Cai, Qinuo"

作者地址："[Liu, Xiang; Lei, Haibo; Hu, Yixiang] Xiangtan Cent Hosp, Dept Clin Pharm, Xiangtan 411100, Peoples R China; [Fan, Xinyao; Zhang, Yazhen; Xie, Liyun; Huang, Jianji; Cai, Qinuo] Chongqing Med Univ, Coll Pharm, Int Med Coll, Chongqing 400016, Peoples R China; [Fan, Xinyao; Zhang, Yazhen; Xie, Liyun; Huang, Jianji; Cai, Qinuo] Chongqing Med Univ, Dept Anesthesiol, Chongqing 400016, Peoples R China"

通信作者："Liu, X (通讯作者)，Xiangtan Cent Hosp, Dept Clin Pharm, Xiangtan 411100, Peoples R China."

来源：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ESI学科分类：CHEMISTRY

WOS号：WOS:001144216700001

JCR分区：Q1

影响因子：4.3

年份：2023

卷号：297

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Hydrogen sulfide (H2S); Inflammation; Gaseous signaling molecule; Nanosensor

摘要："Hydrogen sulfide (H2S) is an endogenously generated gaseous signaling molecule and is known to be involved in the occurrence and development of inflammation. To better understand its physiological and pathological process of inflammation, reliable tools for H2S detection in living inflammatory models are desired. Although a number of fluorescent sensors have been reported for H2S detection and imaging, water-soluble and biocompatibility nanosensors are more useful for imaging in vivo. Herein, we developed a novel biological imaging nanosensor, <bold>XNP1</bold>, for inflammation-targeted imaging of H2S. <bold>XNP1</bold> was obtained by self-assembly of amphiphilic <bold>XNP1</bold>, which was constructed by the condensation reaction of the hydrophobic, H2S response and deep red-emitting fluorophore with hydrophilic biopolymer glycol chitosan (GC). Without H2S, <bold>XNP1</bold> showed very low background fluorescence, while a significant enhancement in the fluorescence intensity of <bold>XNP1</bold> was observed in the presence of H2S, resulting in a high sensitivity toward H2S in aqueous solution with a practical detection limit as low as 32.3 nM, which could be meet the detection of H2S in vivo. <bold>XNP1</bold> also has a good linear response concentration range (0-1 mu M) toward H2S with high selectivity over other competing species. These characteristics facilitate direct H2S detection of the complex living inflammatory cells and drug-induced inflammatory mice, demonstrating its practical application in biosystems."

基金机构：Hunan Pharmaceutical Society [HN201707]; Health Commission of Hunan Province [B2017167]

基金资助正文：This work was partially supported by Hunan Pharmaceutical Society (HN201707) and The Health Commission of Hunan Province (B2017167) .