Single-cell RNA sequencing reveals the transcriptional heterogeneity of Tbx18-positive cardiac cells during heart development

作者全名："Long, Xianglin; Wei, Jiangjun; Fang, Qinghua; Yuan, Xin; Du, Jianlin"

作者地址："[Long, Xianglin; Wei, Jiangjun; Fang, Qinghua; Du, Jianlin] Chongqing Med Univ, Affiliated Hosp 2, Dept Cardiol, Chongqing 400010, Peoples R China; [Yuan, Xin] Chongqing Med Univ, Dept Nephrol, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Du, JL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Cardiol, Chongqing 400010, Peoples R China.; Yuan, X (通讯作者)，Chongqing Med Univ, Dept Nephrol, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：FUNCTIONAL & INTEGRATIVE GENOMICS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001147942000002

JCR分区：Q2

影响因子：2.9

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：ScRNA-seq; Transcriptional heterogeneity; Tbx18; Cardiac cell; Heart development

摘要："The T-box family transcription factor 18 (Tbx18) has been found to play a critical role in regulating the development of the mammalian heart during the primary stages of embryonic development while the cellular heterogeneity and landscape of Tbx18-positive (Tbx18+) cardiac cells remain incompletely characterized. Here, we analyzed prior published single-cell RNA sequencing (scRNA-seq) mouse heart data to explore the heterogeneity of Tbx18+ cardiac cell subpopulations and provide a comprehensive transcriptional landscape of Tbx18+ cardiac cells during their development. Bioinformatic analysis methods were utilized to identify the heterogeneity between cell groups. Based on the gene expression characteristics, Tbx18+ cardiac cells can be classified into a minimum of two distinct cell populations, namely fibroblast-like cells and cardiomyocytes. In terms of temporal heterogeneity, these cells exhibit three developmental stages, namely the MEM stage, ML_P0 stage, and P stage Tbx18+ cardiac cells. Furthermore, Tbx18+ cardiac cells encompass several cell types, including cardiac progenitor-like cells, cardiomyocytes, and epicardial/stromal cells, as determined by specific transcriptional regulatory networks. The scRNA-seq results revealed the involvement of extracellular matrix (ECM) signals and epicardial epithelial-to-mesenchymal transition (EMT) in the development of Tbx18+ cardiac cells. The utilization of a lineage-tracing model served to validate the crucial function of Tbx18 in the differentiation of cardiac cells. Consequently, these findings offer a comprehensive depiction of the cellular heterogeneity within Tbx18+ cardiac cells."

基金机构：Natural Science Foundation of Chongqing Science and Technology Commission [cstc2020jcyj-msxmX0210]; Future Medicine Youth Innovation Team Development Support Program of Chongqing Medical University [W0133]; High-End Medical Talents Project of Middle-Aged and Young People in Chongqing ([2022]); Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University

基金资助正文："This work was supported by grants from the Natural Science Foundation of Chongqing Science and Technology Commission (cstc2020jcyj-msxmX0210), Future Medicine Youth Innovation Team Development Support Program of Chongqing Medical University (W0133), High-End Medical Talents Project of Middle-Aged and Young People in Chongqing (JianlinDu [2022]) and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University."