Adipose-derived mesenchymal stem cells (MSCs) are a superior cell source for bone tissue engineering

作者全名："Gou, Yannian; Huang, Yanran; Luo, Wenping; Li, Yanan; Zhao, Piao; Zhong, Jiamin; Dong, Xiangyu; Guo, Meichun; Li, Aohua; Hao, Ailing; Zhao, Guozhi; Wang, Yonghui; Zhu, Yi; Zhang, Hui; Shi, Yunhan; Wagstaff, William; Luu, Hue H.; Shi, Lewis L.; Reid, Russell R.; He, Tong-Chuan; Fan, Jiaming"

作者地址："[Gou, Yannian; Huang, Yanran; Zhao, Piao; Zhong, Jiamin; Dong, Xiangyu; Guo, Meichun; Li, Aohua; Hao, Ailing; Fan, Jiaming] Chongqing Med Univ, Minist Educ, Key Lab Diagnost Med, Chongqing 400016, Peoples R China; [Gou, Yannian; Huang, Yanran; Zhao, Piao; Zhong, Jiamin; Dong, Xiangyu; Guo, Meichun; Li, Aohua; Hao, Ailing; Fan, Jiaming] Chongqing Med Univ, Sch Lab Med, Dept Clin Biochem, Chongqing 400016, Peoples R China; [Gou, Yannian; Zhao, Piao; Zhong, Jiamin; Zhao, Guozhi; Wang, Yonghui; Zhu, Yi; Zhang, Hui; Shi, Yunhan; Wagstaff, William; Luu, Hue H.; Shi, Lewis L.; Reid, Russell R.; He, Tong-Chuan] Univ Chicago, Mol Oncol Lab, Dept Orthopaed Surg & Rehabil Med, Med Ctr, Chicago, IL 60637 USA; [Luo, Wenping] Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Luo, Wenping] Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Li, Yanan] Southwest Univ, Lab Anim Ctr, Chongqing 400715, Peoples R China; [Wang, Yonghui] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing 401147, Peoples R China; [Zhu, Yi] Shanghai Jiao Tong Univ, Xinhua Hosp, Dept Geriatr, Sch Med, Shanghai 200000, Peoples R China; [Zhang, Hui] Chinese Acad Med Sci & Peking Union Med Coll, Beijing Hosp, Dept Orthopaed Surg, Beijing 100730, Peoples R China; [Shi, Yunhan] Chongqing Univ, Breast Canc Ctr, Canc Hosp, Chongqing 4000430, Peoples R China; [Shi, Yunhan] Univ Rochester, Dept Psychol, Sch Arts & Sci, Rochester, NY 14627 USA; [Reid, Russell R.; He, Tong-Chuan] Univ Chicago, Dept Surg, Med Ctr, Chicago, IL 60637 USA; [Reid, Russell R.; He, Tong-Chuan] Univ Chicago, Lab Craniofacial Biol & Dev, Dept Surg Sect Plast Surg, Med Ctr, Chicago, IL 60637 USA"

通信作者："Fan, JM (通讯作者)，Chongqing Med Univ, Minist Educ, Key Lab Diagnost Med, Chongqing 400016, Peoples R China.; He, TC (通讯作者)，Univ Chicago, Mol Oncol Lab, Dept Orthopaed Surg & Rehabil Med, Med Ctr, Chicago, IL 60637 USA."

来源：BIOACTIVE MATERIALS

ESI学科分类：　

WOS号：WOS:001150360300001

JCR分区：Q1

影响因子：18

年份：2024

卷号：34

期号：　

开始页：51

结束页：63

文献类型：Article

关键词：Mesenchymal stem cell (MSC); Bone tissue engineering; Multipotent progenitor cells; Adipose-derived mesenchymal stem cells; Osteogenic differentiation; Adipogenesis

摘要："Effective bone regeneration through tissue engineering requires a combination of osteogenic progenitors, osteoinductive biofactors and biocompatible scaffold materials. Mesenchymal stem cells (MSCs) represent the most promising seed cells for bone tissue engineering. As multipotent stem cells that can self-renew and differentiate into multiple lineages including bone and fat, MSCs can be isolated from numerous tissues and exhibit varied differentiation potential. To identify an optimal progenitor cell source for bone tissue engineering, we analyzed the proliferative activity and osteogenic potential of four commonly-used mouse MSC sources, including immortalized mouse embryonic fibroblasts (iMEF), immortalized mouse bone marrow stromal stem cells (imBMSC), immortalized mouse calvarial mesenchymal progenitors (iCAL), and immortalized mouse adipose-derived mesenchymal stem cells (iMAD). We found that iMAD exhibited highest osteogenic and adipogenic capabilities upon BMP9 stimulation in vitro, whereas iMAD and iCAL exhibited highest osteogenic capability in BMP9-induced ectopic osteogenesis and critical-sized calvarial defect repair. Transcriptomic analysis revealed that, while each MSC line regulated a distinct set of target genes upon BMP9 stimulation, all MSC lines underwent osteogenic differentiation by regulating osteogenesis-related signaling including Wnt, TGF-beta, PI3K/AKT, MAPK, Hippo and JAK-STAT pathways. Collectively, our results demonstrate that adipose-derived MSCs represent optimal progenitor sources for cell-based bone tissue engineering."

基金机构："Natural Science Foundation of China [82102696]; Chongqing Bayu Young Scholar Award; The 2019 Chongqing Support Program for Entrepreneurship and Innovation [cx2019113]; The 2019 Funding for Postdoctoral Research (Chongqing Human Resources and Social Security Bureau) [298]; Medical Scientist Training Program of the National Institutes of Health [UL1TR002389-07]; University of Chicago Cancer Center Support Grant [CA226303]; National Center for Advancing Translational Sciences of the National Institutes of Health [DE030480]; Mabel Green Myers Research Endowment Fund; University of Chicago Orthopaedics Alumni Fund; National Institutes of Health [T32 GM007281, P30CA014599]"

基金资助正文："The reported study was supported in part by research grants from the Natural Science Foundation of China (82102696 to JF) , the Chongqing Bayu Young Scholar Award (JF) , the 2019 Chongqing Support Program for Entrepreneurship and Innovation (No. cx2019113 to JF) , the 2019 Funding for Postdoctoral Research (Chongqing Human Resources and Social Security Bureau No.298 to JF) , and the National Institutes of Health (CA226303 to TCH, and DE030480 to RRR) . WW was supported by the Medical Scientist Training Program of the National Institutes of Health (T32 GM007281) . This project was also supported in part by The University of Chicago Cancer Center Support Grant (P30CA014599) and the National Center for Advancing Translational Sciences of the National Institutes of Health through Grant Number UL1TR002389-07. TCH was also supported by the Mabel Green Myers Research Endowment Fund and The University of Chicago Orthopaedics Alumni Fund. Funding sources were not involved in the study design, in the collection, analysis and interpretation of data, in the writing of the report, and in the decision to submit the paper for publication."