Network pharmacology analysis and experimental validation to explore the effect and mechanism of tetramethylpyrazine for spinal cord injury

作者全名："Qi, Guodong; Li, Shujun; Jiang, Qiong; Yu, Zhijuan; Peng, Zhenggang; Li, Qiurui; Qi, Wei; Guo, Mingjun"

作者地址："[Qi, Guodong; Peng, Zhenggang; Qi, Wei; Guo, Mingjun] Chongqing Orthoped Hosp Tradit Chinese Med, Orthoped Dept, Chongqing, Peoples R China; [Li, Shujun; Jiang, Qiong; Li, Qiurui] Chongqing Med Univ, Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing, Peoples R China; [Yu, Zhijuan] Chongqing Erlang Community Hlth Serv Ctr, Clin Lab, Chongqing, Peoples R China"

通信作者："Qi, W; Guo, MJ (通讯作者)，Chongqing Orthoped Hosp Tradit Chinese Med, Orthoped Dept, Chongqing, Peoples R China."

来源：JOURNAL OF CHEMICAL NEUROANATOMY

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:001154721800001

JCR分区：Q3

影响因子：2.7

年份：2024

卷号：136

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Spinal cord compression; Ligustrazine; Bioinformatics analysis; Mouse model; MAPK signaling pathways; PI3K/AKT signaling pathways; Neuroprotective

摘要："Objective: To investigate the effect and mechanism of Tetramethylpyrazine (TMP) in treating Spinal Cord Injury (SCI) using network pharmacology analysis and animal experiments. Methods: This study was based on public databases, including PharmMapper, BATMAN-TCM, and STRING, as well as KEGG pathway analysis and other methods of network pharmacology were used to preliminarily explore the molecular mechanism of TMP in the treatment of SCI. Using a mouse SCI compression injury model, the efficacy of TMP was evaluated, and the expression of predictive targets on the PI3K/AKT and MAPK signaling pathways was measured using Western blotting and q-PCR. Results: Network pharmacology analysis showed that TMP may exert therapeutic effects through the MAPK and PI3K/AKT signaling pathways. In animal experimental validation studies, it was shown that after treatment with TMP, the hind limb motor function scores and ramp test scores of the TMP-treated mice improved significantly. HE staining showed that after treatment with TMP, cavities decreased, fewer glial cells proliferated, and fewer inflammatory cells infiltrated; Nielsen staining showed less neuronal loss. Western blot studies showed that compared with the model group, expression of RAS, ERK1/2, RAF1, PI3K, and p-AKT proteins in the spinal cord tissue of mice treated with high-dose TMP was significantly lower. Accordingly, q-PCR studies showed that compared with the model group, the expression levels of RAS, ERK1/2, RAF1, PI3K, and p-AKT genes in the spinal cords of mice in the high-dose TMP group were significantly lower. Conclusion: TMP exhibits a good neuroprotective effect after SCI, which may be related to inhibition of the MAPK and PI3K/AKT signaling pathways."

基金机构："Natural Science Foundation of Chongqing, China [cstc2021jcyj-msxmX0516]; Chongqing Key Specialty Construction Project of Traditional Chinese Medicine; [17]"

基金资助正文："This were sponsored by Natural Science Foundation of Chongqing, China under grant cstc2021jcyj-msxmX0516 (Guodong Qi) and Chongqing Key Specialty Construction Project of Traditional Chinese Medicine under grant Yu TCM [2023] No.17 (Mingjun Guo) ."