FPR1 Antagonist (BOC-MLF) Inhibits Amniotic Epithelial-mesenchymal Transition

作者全名："Huang, Xiao-mei; Liao, E.; Liao, Jun-qun; Liu, Ya-ling; Shao, Yong"

作者地址："[Huang, Xiao-mei; Shao, Yong] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing 400042, Peoples R China; [Liao, E.] Maternal & Child Hlth Hosp Hubei Prov, Dept Obstet, Wuhan 430070, Peoples R China; [Liao, Jun-qun] Chongqing Med Univ, Med Lab Sci, Affiliated Hosp 1, Chongqing 400042, Peoples R China; [Liu, Ya-ling] Yubei Matern & Child Healthcare Hosp, Dept Obstet, Chongqing 400042, Peoples R China"

通信作者："Shao, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing 400042, Peoples R China."

来源：CURRENT MEDICAL SCIENCE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001154980500001

JCR分区：Q3

影响因子：2.4

年份：2024

卷号：44

期号：1

开始页：187

结束页：194

文献类型：Article

关键词：formyl peptide receptor 1; BOC-MLF; epithelial-mesenchymal transition; premature rupture of membranes

摘要："ObjectivePremature rupture of membranes (PROM) is a common pregnancy disorder that is closely associated with structural weakening of fetal membranes. Studies have found that formyl peptide receptor 1 (FPR1) activates inflammatory pathways and amniotic epithelialmesenchymal transition (EMT), stimulates collagen degradation, and leads to membrane weakening and membrane rupture. The purpose of this study was to investigate the anti-inflammatory and EMT inhibitory effects of FPR1 antagonist (BOC-MLF) to provide a basis for clinical prevention of PROM.MethodsThe relationship between PROM, FPR1, and EMT was analyzed in human fetal membrane tissue and plasma samples using Western blotting, PCR, Masson staining, and ELISA assays. Lipopolysaccharide (LPS) was used to establish a fetal membrane inflammation model in pregnant rats, and BOC-MLF was used to treat the LPS rat model. We detected interleukin (IL)-6 in blood from the rat hearts to determine whether the inflammatory model was successful and whether the anti-inflammatory treatment was effective. We used electron microscopy to analyze the structure and collagen expression of rat fetal membrane.ResultsWestern blotting, PCR and Masson staining indicated that the expression of FPR1 was significantly increased, the expression of collagen was decreased, and EMT appeared in PROM. The rat model indicated that LPS caused the collapse of fetal membrane epithelial cells, increased intercellular gaps, and decreased collagen. BOC-MLF promoted an increase in fetal membrane collagen, inhibited EMT, and reduced the weakening of fetal membranes.ConclusionThe expression of FPR1 in the fetal membrane of PROM was significantly increased, and EMT of the amniotic membrane was obvious. BOC-MLF can treat inflammation and inhibit amniotic EMT."

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