Precise quantification of microRNAs based on proximity ligation of AuNPs-immobilized DNA probes

作者全名："Li, Keyu; Xiao, Peng; Yuan, Ningning; Yan, Shujuan; Zhao, Pei; Zuo, Guowei"

作者地址："[Li, Keyu; Xiao, Peng; Yuan, Ningning; Zuo, Guowei] Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med, Chinese Minist Educ, Chongqing 400016, Peoples R China; [Yan, Shujuan] Guangzhou Med Univ, Prenatal Diagnost Ctr, Guangzhou Women & Childrens Med Ctr, Guangzhou 510620, Guangdong, Peoples R China; [Zhao, Pei] Hebei Gen Hosp, Dept Lab Med, Shijiazhuang 050051, Peoples R China"

通信作者："Zuo, GW (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med, Chinese Minist Educ, Chongqing 400016, Peoples R China.; Zhao, P (通讯作者)，Hebei Gen Hosp, Dept Lab Med, Shijiazhuang 050051, Peoples R China."

来源：ANALYTICAL METHODS

ESI学科分类：CHEMISTRY

WOS号：WOS:001157801400001

JCR分区：Q1

影响因子：2.7

年份：2024

卷号：　

期号：　

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结束页：　

文献类型：Article; Early Access

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摘要："MiRNAs are critical regulators of target gene expression in many biological processes and are considered promising biomarkers for diseases. In this study, we developed a simple, specific, and sensitive miRNA detection method based on proximity ligation reaction, which is easy to operate. The method uses a pair of target-specific DNA probes immobilized on the same gold nanoparticles (AuNPs), which hybridize to the target miRNA. Hybridization brings the probes close together, allowing the formation of a continuous DNA sequence that can be amplified by Quantitative Real-time PCR (qPCR). This method eliminates the need for complex reverse transcription design and achieves high specificity for discriminating single base mismatches between miRNAs through a simple procedure. This method can sensitively measure three different miRNAs with a detection limit of 20 aM, providing high versatility and sensitivity, even distinguishing single-base variations among members of the miR-200 family with high selectivity. Due to its high selectivity and sensitivity, this method has important implications for the investigation of miRNA biological functions and related biomedical research. MiRNAs are critical regulators of target gene expression in many biological processes. In this study, we developed a simple, specific, and sensitive miRNA detection method based on proximity ligation reaction of AuNPs-immobilized DNA probes, which is easy to operate."

基金机构：National Natural Science Foundation of China [82060388]; National Natural Science Foundation of China

基金资助正文：Funding was provided by the National Natural Science Foundation of China (82060388).