"Increased NAD plus levels protect female mouse reproductive system against zearalenone-impaired glycolysis, lipid metabolism, antioxidant capacity and inflammation"

作者全名："Dai, Chao; Hou, Mengqian; Yang, Xudong; Wang, Zhefeng; Sun, Changpo; Wu, Xin; Wang, Shujin"

作者地址："[Dai, Chao; Wang, Zhefeng; Wu, Xin] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Peoples R China; [Dai, Chao; Hou, Mengqian; Wang, Shujin] Chongqing Med Univ, Inst Life Sci, Chongqing 400032, Peoples R China; [Dai, Chao; Wang, Zhefeng] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China; [Yang, Xudong] Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Key Lab Agr Anim Breeding & Hlth Husb, Tianjin, Peoples R China; [Wang, Zhefeng; Sun, Changpo] Acad Natl Food, Strateg Reserv Adm, Beijing 100037, Peoples R China; [Sun, Changpo] Strateg Reserv Adm, Stand & Qual Ctr Natl Food, Beijing 100037, Peoples R China; [Wu, Xin] Tianjin Inst Ind Biotechnol, Chinese Acad Sci, Tianjin 300308, Peoples R China"

通信作者："Wu, X (通讯作者)，Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Peoples R China.; Wang, SJ (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing 400032, Peoples R China."

来源：REPRODUCTIVE TOXICOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001157963300001

JCR分区：Q2

影响因子：3.3

年份：2024

卷号：124

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Mycotoxin; Zearalenone; Nicotinamide adenine dinucleotide; Lipid accumulation; Antioxidant capacity; Inflammation

摘要："The reproductive system is a primary target organ for zearalenone (ZEN, a widespread fusarium mycotoxin) to exert its toxic effects, including decreased antioxidant capacity and aggravated inflammatory response. These ZEN-induced reproductive abnormalities are partially caused by the declining levels of nicotinamide adenine dinucleotide (NAD+), which results in an imbalance in lipid/glucose metabolism. Accordingly, the present study aimed to investigate whether supplements of nicotinamide mononucleotide (NMN, a NAD+ precursor) in female mice could protect against ZEN-induced reproductive toxicity. In this study, thirty female mice were randomly divided into three groups that were intragastrically administered with i) 0.5% DMSO (the Ctrl group), ii) 3 mg/ (kg bw.d) ZEN (the ZEN group), or iii) ZEN + 500 mg/(kg bw.d) NMN (the ZEN/NMN group) for two weeks. The results revealed that, compared with the Ctrl group, animals exposed to ZEN exhibited reproductive toxicity, such as decreased antioxidant capacity and aggravated inflammatory response in reproductive tissues. These effects were strongly correlated with lower activities in key glycolytic enzymes (e.g., ALDOA and PGK), but increased expressions in key lipid-synthesis genes (e.g., LPIN1 and ATGL). These changes contribute to lipid accumulation, specifically for diacylglycerols (DAGs). Furthermore, these ZEN-induced changes were linked with disturbed NAD+ synthesis/degradation, and subsequently decreased NAD+ levels. Notably, NMN supplements in mice protected against these ZEN-induced reproductive abnormalities by boosting NAD+ levels. Herein, the present findings demonstrate that potential strategies to enhance NAD+ levels can protect against ZEN-induced reproductive toxicity"

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