A novel electrochemiluminescent cytosensor using 3D multivalent aptamer recognition and covalent organic frameworks-associated DNA walker for highly efficient capture and detection of rare CTCs

作者全名："Guo, Minghui; Shen, Bo; He, Wen; Li, Xinyu; Li, Xinmin; Li, Menghan; Hu, Ruiwei; Zhang, Mengxuan; Yan, Yurong"

作者地址："[Guo, Minghui; He, Wen; Li, Xinyu; Li, Menghan; Hu, Ruiwei; Zhang, Mengxuan; Yan, Yurong] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Shen, Bo; Li, Xinmin] Chongqing Hosp Tradit Chinese Med, Dept Clin Lab, Chongqing 400021, Peoples R China; [Shen, Bo; Li, Xinmin] Chongqing Hosp Tradit Chinese Med, Chongqing Key Lab Sichuan Chongqing Coconstruct Di, Chongqing 400021, Peoples R China"

通信作者："Yan, YR (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:001158560200001

JCR分区：Q1

影响因子：13.3

年份：2024

卷号：481

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Electrochemiluminescence cytosensor; Multivalent aptamer recognition; DNA walker; Covalent organic frameworks; Circulating tumor cells

摘要："Circulating tumor cells (CTCs) are regarded as promising biomarkers for early clinical diagnosis, prognosis evaluation, and treatment monitoring. However, their limited abundance in peripheral blood and phenotypic heterogeneity resulting from epithelial-mesenchymal transition (EMT) present significant challenges for accurate detection of CTCs. Herein, we developed an ultrasensitive and highly specific cytosensor to address these limitations. Firstly, to overcome the EMT property of CTCs, we designed a novel 3D multivalent aptamer recognition strategy (MARS), which not only contributed to highly efficient capture of heterogeneous CTCs, but also guaranteed detection sensitivity of the developed cytosensor. Meanwhile, we integrated an exonuclease III (Exo III)- mediated DNA walking signal amplification strategy into the electrochemiluminescence (ECL) cytosensor, enhancing its sensitivity in detecting rare CTCs. Furthermore, in combination with the excellent ECL performance of the luminescent nanomaterials (Au@COF-LZU1@Ru), the cytosensor exhibited a wide detection range from 8 to 1 x 10(5) cells/mL with a detection limit as low as 2 cells/mL. The analytical performance of the constructed cytosensor was validated through real sample analysis (AUC = 0.97), demonstrating its potential for clinical applications. The novel cytosensor offers an effective and reliable tool for the detection of rare CTCs, thereby paving the way for improved clinical significance."

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