Collagen-Targeting Self-Assembled Nanoprobes for Multimodal Molecular Imaging and Quantification of Myocardial Fibrosis in a Rat Model of Myocardial Infarction

作者全名："Li, Fang; Chen, Lihua; Zhong, Shigeng; Chen, Jinhua; Cao, Yang; Yu, Han; Ran, Haitao; Yin, Yuehui; Reutelingsperger, Chris; Shu, Shiyu; Ling, Zhiyu"

作者地址："[Li, Fang; Chen, Lihua; Yin, Yuehui; Ling, Zhiyu] Chongqing Med Univ, Affiliated Hosp 2, Dept Cardiol, Chongqing 400010, Peoples R China; [Zhong, Shigeng] Chongqing Peoples Hosp, Dept Ultrasound, Chongqing 400010, Peoples R China; [Chen, Jinhua; Yu, Han] Chongqing Med Univ, Affiliated Hosp 2, Dept Radiol, Chongqing 400010, Peoples R China; [Cao, Yang; Ran, Haitao] Chongqing Med Univ, Affiliated Hosp 2, Chongqing Key Lab Ultrasound Mol Imaging, Dept Ultrasound, Chongqing 400010, Peoples R China; [Reutelingsperger, Chris] Univ Maastricht, Cardiovasc Res Inst Maastricht, Dept Biochem, NL-6200 MD Maastricht, Netherlands; [Shu, Shiyu] Chongqing Med Univ, Affiliated Hosp 2, Dept Anesthesiol, Chongqing 400010, Peoples R China"

通信作者："Ling, ZY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Cardiol, Chongqing 400010, Peoples R China.; Shu, SY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Anesthesiol, Chongqing 400010, Peoples R China."

来源：ACS NANO

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001158981000001

JCR分区：Q1

影响因子：17.1

年份：2024

卷号：18

期号：6

开始页：4886

结束页：4902

文献类型：Article

关键词：myocardial fibrosis; multimodal imaging; CNA35; photoacoustic; ultrasound; CT

摘要："Currently, inadequate early diagnostic methods hinder the prompt treatment of patients with heart failure and myocardial fibrosis. Magnetic resonance imaging is the gold standard noninvasive diagnostic method; however, its effectiveness is constrained by low resolution and challenges posed by certain patients who cannot undergo the procedure. Although enhanced computed tomography (CT) offers high resolution, challenges arise owing to the unclear differentiation between fibrotic and normal myocardial tissue. Furthermore, although echocardiography is real-time and convenient, it lacks the necessary resolution for detecting fibrotic myocardium, thus limiting its value in fibrosis detection. Inspired by the postinfarction accumulation of collagen types I and III, we developed a collagen-targeted multimodal imaging nanoplatform, CNA35-GP@NPs, comprising lipid nanoparticles (NPs), encapsulating gold nanorods (GNRs) and perfluoropentane (PFP). This platform facilitated ultrasound/photoacoustic/CT imaging of postinfarction cardiac fibrosis in a rat model of myocardial infarction (MI). The surface-modified peptide CNA35 exhibited excellent collagen fiber targeting. The strong near-infrared light absorption and substantial X-ray attenuation of the nanoplatform rendered it suitable for photoacoustic and CT imaging. In the rat model of MI, our study demonstrated that CNA35-GNR/PFP@NPs (CNA35-GP@NPs) achieved photoacoustic, ultrasound, and enhanced CT imaging of the fibrotic myocardium. Notably, the photoacoustic signal intensity positively correlated with the severity of myocardial fibrosis. Thus, this study presents a promising approach for accurately detecting and treating the fibrotic myocardium."

基金机构："National Natural Science Foundation of China [82170520]; National Natural Science Foundation of China [cstc2021jcyj-msxmX0075]; Natural Science Foundation Project of Chongqing [W0078]; Program for Youth Innovation in Future Medicine, Chongqing Medical University; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China"

基金资助正文："This research is funded by the National Natural Science Foundation of China (Grant 82170520), Natural Science Foundation Project of Chongqing (Grant cstc2021jcyj-msxmX0075), Program for Youth Innovation in Future Medicine, Chongqing Medical University (Grant W0078), and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China."