Au/PANI@PtCu-based electrochemical immunosensor for ultrasensitive determination of pro-gastrin-releasing peptide

作者全名："Fu, Xuhuai; Gao, Ke; Liu, Nanjing; Guo, Bianqin; He, Meng; Lai, Nianyu; Li, Xinyu; Ding, Shijia; He, Xiaoyan; Wu, Lixiang"

作者地址："[Fu, Xuhuai; Liu, Nanjing; Guo, Bianqin; He, Meng; Lai, Nianyu; Wu, Lixiang] Chongqing Univ, Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Dept Clin Lab, Chongqing 400030, Peoples R China; [Fu, Xuhuai; Liu, Nanjing; Guo, Bianqin; He, Meng; Lai, Nianyu; Wu, Lixiang] Chongqing Canc Inst, Chongqing 400030, Peoples R China; [Gao, Ke] Chonggang Gen Hosp, Dept Lab Med, Chongqing 400080, Peoples R China; [Li, Xinyu; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [He, Xiaoyan] Chongqing Med Univ, Minist Educ,Key Lab Child Dev & Disorders, Ctr Clin Mol Med,Natl Clin Res Ctr Child Hlth & Di, Chongqing Key Lab Pediat,Childrens Hosp,China Int, Chongqing 400014, Peoples R China"

通信作者："Wu, LX (通讯作者)，Chongqing Univ, Canc Hosp, Chongqing Key Lab Translat Res Canc Metastasis & I, Dept Clin Lab, Chongqing 400030, Peoples R China.; Wu, LX (通讯作者)，Chongqing Canc Inst, Chongqing 400030, Peoples R China.; He, XY (通讯作者)，Chongqing Med Univ, Minist Educ,Key Lab Child Dev & Disorders, Ctr Clin Mol Med,Natl Clin Res Ctr Child Hlth & Di, Chongqing Key Lab Pediat,Childrens Hosp,China Int, Chongqing 400014, Peoples R China."

来源：MICROCHIMICA ACTA

ESI学科分类：CHEMISTRY

WOS号：WOS:001159342900001

JCR分区：Q1

影响因子：5.3

年份：2024

卷号：191

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：Au/PANI@PtCu; Electro-catalytic activity; Electrochemical immunosensor; Modified glassy carbon electrode; Multi-wall carbon nanotubes; Differential pulse voltammetry

摘要："An ultrasensitive sandwich-type electrochemical immunosensor for pro-gastrin-releasing peptide (ProGRP) detection was constructed based on PtCu nanodendrites functionalized Au/polyaniline nanospheres (Au/PANI@PtCu). The prepared Au/PANI@PtCu nanocomposites not only possessed excellent electro-catalytic activity of H2O2 reduction due to the synergistic effect between the Au/PANI and PtCu NDs but also provided large specific surface area for detection of antibodies (Ab2) immobilization. In addition, Au nanoparticles encapsulated multi-wall carbon nanotubes (AuNPs@MWCNTs) were also applied to modify the glassy carbon electrode interface for loading numerous capture antibodies (Ab1). In the presence of target ProGRP, a sandwich-type electrochemical immunosensor showed a strong current response from the electro-catalysis of Au/PANI@PtCu toward H2O2 reduction. Benefiting from the exceptional electro-catalytic performance of Au/PANI@PtCu and the high conductivity of AuNPs@MWCNTs, the sandwich-type immunoassay exhibited remarkable sensitivity in detection. The linear range extended from 100 fg/mL to 10 ng/mL, while achieving an impressively low limit of detection of 77.62 fg/mL."

基金机构：Chongqing Bureau of Science and Technology and Chongqing Municipal Health Commission joint research project [2021MSXM167]

基金资助正文：This study was supported by the Chongqing Bureau of Science and Technology and Chongqing Municipal Health Commission joint research project (Grant Number 2021MSXM167).