p75NTR antibody-conjugated microspheres: an approach to guided tissue regeneration by selective recruitment of endogenous periodontal ligament cells

作者全名："Zou, Xuqiang; Xie, Bo; Peng, Xuelian; Lu, Mingjie; Xu, Dan; Yuan, Hongyan; Zhang, Yixin; Wang, Di; Zhao, Manzhu; Liu, Rui; Wen, Xiujie"

作者地址："[Zou, Xuqiang; Xie, Bo; Peng, Xuelian; Lu, Mingjie; Xu, Dan; Zhang, Yixin; Wen, Xiujie] Southwest Med Univ, Sch Stomatol, Dept Orthodont, Luzhou, Peoples R China; [Yuan, Hongyan; Wang, Di; Zhao, Manzhu] Chongqing Med Univ, Coll Stomatol, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Liu, Rui] Third Mil Med Univ, Army Med Univ, Daping Hosp, Dept Stomatol, Chongqing, Peoples R China"

通信作者："Wen, XJ (通讯作者)，Southwest Med Univ, Sch Stomatol, Dept Orthodont, Luzhou, Peoples R China.; Liu, R (通讯作者)，Third Mil Med Univ, Army Med Univ, Daping Hosp, Dept Stomatol, Chongqing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:001160952400001

JCR分区：Q1

影响因子：5.7

年份：2024

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：p75NTR; antibody conjugation; periodontal ligament cells; chitosan microspheres; nano-hydroxyapatite; cell recruitment

摘要："Repairing defects in alveolar bone is essential for regenerating periodontal tissue, but it is a formidable challenge. One promising therapeutic approach involves using a strategy that specifically recruits periodontal ligament cells (PDLCs) with high regenerative potential to achieve in situ regeneration of alveolar bone. In this study, we have created a new type of microsphere conjugated with an antibody to target p75 neurotrophin receptor (p75NTR), which is made of nano-hydroxyapatite (nHA) and chitosan (CS). The goal of this design is to attract p75NTR+hPDLCs selectively and promote osteogenesis. In vitro experiments demonstrated that the antibody-conjugated microspheres attracted significantly more PDLCs compared to non-conjugated microspheres. Incorporating nHA not only enhances cell adhesion and proliferation on the surface of the microsphere but also augments its osteoinductive properties. Microspheres effectively recruited p75NTR+ cells at bone defect sites in SD rats, as observed through immunofluorescent staining of p75NTR antibodies. This p75NTR antibody-conjugated nHA/CS microsphere presents a promising approach for selectively recruiting cells and repairing bone defects."

基金机构：National Natural Science Foundation of China10.13039/501100001809; Public Platform of Cell and Molecular Biotechnology (Southwest Medical University)

基金资助正文：We appreciate the great help from the Public Platform of Cell and Molecular Biotechnology (Southwest Medical University).