Dual-monomer solvatochromic probe system (DSPS) for effectively differentiating lipid raft cholesterol and active membrane cholesterol in the inner-leaflet plasma membrane

作者全名："Luo, Xuan; Zhao, Yunfei; Cai, Yang; Chen, Jun; Zhao, Lulu; Lan, Tianlan; Chen, Yaxi; Ruan, Xiong Z."

作者地址："[Luo, Xuan; Lan, Tianlan; Chen, Yaxi; Ruan, Xiong Z.] Chongqing Med Univ, Affiliated Hosp 2, Ctr Lipid Res, Chongqing 400016, Peoples R China; [Luo, Xuan; Lan, Tianlan; Chen, Yaxi; Ruan, Xiong Z.] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Chongqing Key Lab Metab Lipid & Gl, Chongqing, Peoples R China; [Zhao, Yunfei; Cai, Yang; Zhao, Lulu] Chongqing Med Univ, Coll Pharm, Chongqing Pharmacodynam Evaluat Engn Technol Res C, Chongqing Key Lab Pharmaceut Metab Res, Chongqing 400016, Peoples R China; [Chen, Jun] Chongging Med Univ, Women & Children Hosp, Dept Pediat, Chongqing 400016, Peoples R China; [Ruan, Xiong Z.] UCL, Med Sch, Ctr Nephrol, John Moorhead Res Lab, Royal Free Campus, London NW3 2PF, England"

通信作者："Chen, YX; Ruan, XZ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Ctr Lipid Res, Chongqing 400016, Peoples R China.; Chen, YX; Ruan, XZ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Chongqing Key Lab Metab Lipid & Gl, Chongqing, Peoples R China.; Ruan, XZ (通讯作者)，UCL, Med Sch, Ctr Nephrol, John Moorhead Res Lab, Royal Free Campus, London NW3 2PF, England."

来源：JOURNAL OF MATERIALS CHEMISTRY B

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001162878200001

JCR分区：Q1

影响因子：6.1

年份：2024

卷号：12

期号：10

开始页：2547

结束页：2558

文献类型：Article

关键词：　

摘要："Monitoring active membrane cholesterol and lipid raft cholesterol in the inner leaflet of the plasma membrane is significant for understanding the membrane function and cellular physiopathological processes. Limited by existing methods, it is difficult to differentiate active membrane cholesterol and lipid raft cholesterol. A novel dual-monomer solvatochromic probe system (DSPS) that targets two types of cholesterol was developed. Acrylodan-BG/SNAP-D4 composed of SNAP-D4 cholesterol-recognizing monomers and solvatochromic acrylodan-BG-sensing monomers exhibits excellent cholesterol detecting properties in terms of selectivity, accuracy, convenience and economic benefits. Cell imaging revealed that lipid raft cholesterol emitted blue fluorescence, whereas active membrane cholesterol (which partially bobbed in aqueous cytosol) displayed green fluorescence; both the fluorescence emissions increased or decreased in a cholesterol-dependent manner. This system provides a new technology for the determination of two types of cholesterol, which is beneficial for the further study of membrane function, intracellular cholesterol trafficking, and cell signaling. A novel, selective, practical cholesterol sensing system based on a fusion protein and a solvatochromic molecule was developed to distinguish cholesterol exposed in different phases of the plasma membrane."

基金机构："National Natural Science Foundation of China [2022YFC2502500]; National Key R&D Program of China [32030054, 82170586, U23A20415]; National Natural Science Foundation of China [KJZD-K202200402, CSTB2023NSCQ-LZX0001]; Science and Technology Research Program of Chongqing Municipal Education Commission; Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University"

基金资助正文："This work was supported by National Key R&D Program of China (2022YFC2502500); the National Natural Science Foundation of China (32030054, 82170586, U23A20415); the Science and Technology Research Program of Chongqing Municipal Education Commission (grant no. KJZD-K202200402, CSTB2023NSCQ-LZX0001); Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University."