Comprehensive analysis of the gut microbiome and post- translational modifications elucidates the route involved in microbiota-host interactions

作者全名："Wang, Hai-Yang; Liu, Lan-Xiang; Chen, Xue-Yi; Zhang, Yang-Dong; Li, Wen-Xia; Li, Wen-Wen; Wang, Lian; Mo, Xiao- Long; Wei, Hong; Ji, Ping; Xie, Peng"

作者地址："[Wang, Hai-Yang; Li, Wen-Xia; Wang, Lian; Mo, Xiao- Long; Xie, Peng] Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Wang, Hai-Yang; Ji, Ping; Xie, Peng] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China; [Wang, Hai-Yang; Ji, Ping; Xie, Peng] Chongqing Med Univ, Affiliated Stomatol Hosp, Chongqing 401147, Peoples R China; [Wang, Hai-Yang; Ji, Ping] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China; [Liu, Lan-Xiang] Chongqing Med Univ, Dept Neurol, Yongchuan Hosp, Chongqing 402160, Peoples R China; [Chen, Xue-Yi; Li, Wen-Wen] Chongqing Med Univ, Fac Basic Med, Dept Pathol, Chongqing 400016, Peoples R China; [Zhang, Yang-Dong; Mo, Xiao- Long; Xie, Peng] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Wei, Hong] Yu Yue Pathol Sci Res Ctr, Chongqing 408201, Peoples R China"

通信作者："Xie, P (通讯作者)，Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Ji, P; Xie, P (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China.; Ji, P; Xie, P (通讯作者)，Chongqing Med Univ, Affiliated Stomatol Hosp, Chongqing 401147, Peoples R China.; Ji, P (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China.; Xie, P (通讯作者)，Chongqing Med Univ, Dept Neurol, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Wei, H (通讯作者)，Yu Yue Pathol Sci Res Ctr, Chongqing 408201, Peoples R China."

来源：ZOOLOGICAL RESEARCH

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:001165342100001

JCR分区：Q1

影响因子：4.9

年份：2024

卷号：45

期号：1

开始页：95

结束页：107

文献类型：Article

关键词：Gut microbiota; Hippocampal protein; Post-translational modifications; Succinylation; Acetylation; Phosphorylation

摘要："The gut microbiome interacts with the host to maintain body homeostasis, with gut microbial dysbiosis implicated in many diseases. However, the underlying mechanisms of gut microbe regulation of host behavior and brain functions remain unclear. This study aimed to elucidate the influence of gut microbiota on brain functions via post-translational modification mechanisms in the presence or absence of bacteria without any stimulation. We conducted succinylome analysis of hippocampal proteins in germ-free (GF) and specific pathogen-free (SPF) mice and metagenomic analysis of feces from SPF mice. These results were integrated with previously reported hippocampal acetylome and phosphorylome data from the same batch of mice. Subsequent bioinformatics analyses revealed 584 succinylation sites on 455 proteins, including 54 up-regulated succinylation sites on 91 proteins and 99 down-regulated sites on 51 proteins in the GF mice compared to the SPF mice. We constructed a panoramic map of gut microbiota-regulated succinylation, acetylation, and phosphorylation, and identified cross-talk and relative independence between the different types of post-translational modifications in modulating complicated intracellular pathways. Pearson correlation analysis indicated that 13 taxa, predominantly belonging to the Bacteroidetes phylum, were correlated with the biological functions of post-translational modifications. Positive correlations between these taxa and succinylation and negative correlations between these taxa and acetylation were identified in the modulation of intracellular pathways. This study highlights the hippocampal physiological changes induced by the absence of gut microbiota, and proteomic quantification of succinylation, phosphorylation, and acetylation, contributing to our understanding of the role of the gut microbiome in brain function and behavioral phenotypes."

基金机构："Natural Science Foundation Project of China [81820108015, 82201683]; China Postdoctoral Science Foundation [2021M693926, 2020TQ0393, 2020M683634XB]; Chongqing Science & Technology Commission [cstc2021jcyj-bshX0150, cstc2021jcyj-bshX0201]; Special Funding for Chongqing Postdoctoral Research Projects [2021XMT001]"

基金资助正文："This work was supported by the Natural Science Foundation Project of China (81820108015, 82201683) , China Postdoctoral Science Foundation (2021M693926, 2020TQ0393, 2020M683634XB) , Chongqing Science & Technology Commission (cstc2021jcyj-bshX0150, cstc2021jcyj-bshX0201) , and Special Funding for Chongqing Postdoctoral Research Projects (2021XMT001)"