A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-D-aspartate receptor antibodies via molecular mimicry in mice

作者全名："Luo, Hanyu; Yang, Jiaxin; Yang, Xiaoyue; Han, Ziyao; Fang, Zhixu; Huang, Dishu; Gui, Jianxiong; Ding, Ran; Chen, Hengsheng; Cheng, Li; Ma, Jiannan; Jiang, Li"

作者地址："[Luo, Hanyu; Yang, Jiaxin; Yang, Xiaoyue; Han, Ziyao; Fang, Zhixu; Huang, Dishu; Gui, Jianxiong; Ding, Ran; Chen, Hengsheng; Cheng, Li; Ma, Jiannan; Jiang, Li] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Dept Neurol,Childrens Hosp,Minist Educ, Chongqing, Peoples R China; [Luo, Hanyu; Yang, Jiaxin; Yang, Xiaoyue; Han, Ziyao; Fang, Zhixu; Huang, Dishu; Gui, Jianxiong; Ding, Ran; Chen, Hengsheng; Cheng, Li; Ma, Jiannan; Jiang, Li] Chongqing Key Lab Child Neurodev & Cognit Disorder, Chongqing, Peoples R China; [Ma, Jiannan; Jiang, Li] 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China"

通信作者："Ma, JN; Jiang, L (通讯作者)，136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:001166304300001

JCR分区：Q2

影响因子：4

年份：2024

卷号：10

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：Anti-NMDAR encephalitis; Japanese encephalitis; Active immunization; Molecular mimicry; Peptide

摘要："Background: The development of anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis following viral encephalitis, such as Japanese encephalitis, has received increasing attention in recent years. However, the mechanism of anti-NMDAR antibody production following Japanese encephalitis has not been explored. Methods: A peptide from the Japanese encephalitis virus (JEV), which shares a similar amino acid sequence with GluN1, was identified by sequence comparison. We then explored whether active subcutaneous immunization with the JEV peptide could induce the production of anti-NMDAR antibodies and related pathophysiological and behavioral changes in mice. In addition, a published active immune model of anti-NMDAR encephalitis using a GluN1 peptide was used as the positive control. Results: A 6-amino-acid sequence with 83 % similarity between the envelope protein of the JEV (HGTVVI) and GluN1 (NGTHVI) was identified, and the sequence included the N368/G369 region. Active immunization with the JEV peptide induced a substantial and specific immune response in mice. However, anti-NMDAR antibodies were not detected in the serum of mice immunized with the JEV peptide by ELISA, CBA, and TBA. Moreover, mice immunized with the JEV peptide presented no abnormities related to anti-NMDAR antibodies according to western blotting, patch clamp, and a series of behavioral tests. In addition, active immunization with a recently reported GluN1 peptide failed to induce anti-NMDAR antibody production in mice. Conclusions: In this study, the attempt of active immunization with the JEV peptide to induce the production of anti-NMDAR antibodies via molecular mimicry failed. The pathogenesis of antiNMDAR encephalitis following Japanese encephalitis remains to be elucidated."

基金机构："Natural Science Foundation of Chongqing, China [cstc2021jcyj-msxmX0228]; Chongqing Postgraduate Research and Innovation Project [CYB21184]"

基金资助正文："<BOLD>Funding</BOLD> This work was sponsored by the Natural Science Foundation of Chongqing, China (grant number: cstc2021jcyj-msxmX0228) and the Chongqing Postgraduate Research and Innovation Project (grant number: CYB21184) ."