MZB1-expressing cells are essential for local immunoglobulin production in chronic rhinosinusitis with nasal polyps

作者全名："Huang, Yanran; Xu, Zhaofeng; Holtappels, Gabriele; Shen, Yang; Van Zele, Thibaut; Wen, Weiping; Zhang, Luo; Zhang, Nan; Bachert, Claus"

作者地址："[Huang, Yanran; Xu, Zhaofeng; Wen, Weiping; Bachert, Claus] Sun Yat Sen Univ, Affiliated Hosp 1, Int Airway Res Ctr, Dept Otorhinolaryngol, Guangzhou, Peoples R China; [Huang, Yanran; Zhang, Luo] Capital Med Univ, Beijing TongRen Hosp, Dept Allergy & Otolaryngol Head & Neck Surg, Beijing, Peoples R China; [Huang, Yanran; Xu, Zhaofeng; Holtappels, Gabriele; Van Zele, Thibaut; Zhang, Nan; Bachert, Claus] Univ Ghent, Upper Airway Res Lab, Ghent, Belgium; [Huang, Yanran; Zhang, Luo] Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, 17 HouGouHuTong, Beijing 100005, Peoples R China; [Shen, Yang] Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Chongqing, Peoples R China; [Wen, Weiping] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Otorhinolaryngol, Div Head & Neck Surg, Guangzhou, Peoples R China; [Bachert, Claus] Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Ear Nose & Throat Dis, Stockholm, Sweden; [Bachert, Claus] Univ Clin Munster, Clin ENT Dis & Head & Neck Surg, Munster, Germany; [Zhang, Nan] Univ Hosp Ghent, Upper Airways Res Lab, Corneel Heymanslaan 10, B-9000 Ghent, Belgium"

通信作者："Zhang, L (通讯作者)，Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, 17 HouGouHuTong, Beijing 100005, Peoples R China.; Zhang, N (通讯作者)，Univ Hosp Ghent, Upper Airways Res Lab, Corneel Heymanslaan 10, B-9000 Ghent, Belgium."

来源：ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001170464500001

JCR分区：Q1

影响因子：5.8

年份：2024

卷号：132

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Background: The expression of MZB1 genes is significantly elevated in patients who have chronic rhinosinusitis with nasal polyp (CRSwNP) disease compared with healthy controls. Objective: To characterize MZB1-positive B cells in CRSwNP and to estimate the contribution of distinct subsets of B cells to the local overproduction of immunoglobulins. Methods: Single-cell RNA-sequencing with Cellular Indexing of Transcriptomes and Epitopes by Sequencing technology, Switching Mechanism At the 50 end of RNA Template sequencing, flow cytometry, immunohistochemistry and immunofluorescence staining, Western blot, QuantiGene Plex assay, B-cell ImmunoSpot assay, Luminex assay, and enzyme-linked immunosorbent assay were performed. Results: Significantly higher mRNA expression of MZB1 and HSP90B1 was found in type 2 CRSwNP compared with controls. In CRSwNP, MZB1 expression correlated with the local production of IgE. MZB1 could be colocalized with plasma and mature B cells, especially marginal zone (MZ) B cells. Single-cell transcriptome and epitope studies revealed prominent populations of B cells in type 2 CRSwNP with unexpectedly high MZB1 gene expression. The MZ B-cell population was significantly increased in CRSwNP compared with healthy controls in both peripheral blood mononuclear cells and nasal tissue single-cell suspensions. When those single cells were cultured overnight, the MZ B-cell numbers were positively correlated with local IgE production but negatively correlated with local IgM production. In vitro, MZB1 stimulation up-regulated the mRNA expression of IgE. Conclusion: MZB1 was primarily expressed by plasma and mature B cells in nasal mucosa. MZB1 expression level was increased in CRSwNP compared with controls. MZB1 contributed to the local IgE production in type 2 CRSwNP. (c) 2023 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved."

基金机构："Chinese Scholarship Council [202008110300]; Chinese Postdoctoral Science Foundation [2022M723611]; National Natural Science Foundation of People's Republic of China [82301272, 82301282]; FWO Flanders FWO-EOS Project [G0G2318N]; Interuniversity Attraction Poles Grant [P7/30]; Guangzhou Municipal Science and Technology Program [2023A04J2201]"

基金资助正文："This study was supported by the Chinese Scholarship Council fellowship (202008110300) , Chinese Postdoctoral Science Foundation (2022M723611) , National Natural Science Foundation of People's Republic of China (82301272, 82301282) , FWO Flanders FWO-EOS Project (G0G2318N) , The Interuniversity Attraction Poles Grant (P7/30) , and the Guangzhou Municipal Science and Technology Program (Affiliation: Guangzhou Municipal Science and Technology Bureau, 2023A04J2201) ."