Long Noncoding RNA GAS5-Involved Progression of Neonatal Hydrocephalus and Inflammatory Responses

作者全名："Zou, Bin; Zhang, Qin; Gan, Hui; Qin, Yue; Zhou, Yudong; Zhai, Xuan; Liang, Ping"

作者地址："[Zou, Bin; Zhou, Yudong; Zhai, Xuan; Liang, Ping] Chongqing Med Univ, Childrens Hosp,Dept Neurosurg, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ,Key Lab Child Dev & Disorders,Chongqin, 20 Jinyu Rd, Chongqing 401122, Peoples R China; [Zhang, Qin] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Cardiol,Childrens Hosp, Minist Educ,Key Lab Child Dev & Disorders, Chongqing City 401122, Peoples R China; [Gan, Hui; Qin, Yue] Chongqing Med Univ, Chongqing City 400016, Peoples R China"

通信作者："Liang, P (通讯作者)，Chongqing Med Univ, Childrens Hosp,Dept Neurosurg, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ,Key Lab Child Dev & Disorders,Chongqin, 20 Jinyu Rd, Chongqing 401122, Peoples R China."

来源：MOLECULAR BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001171457500001

JCR分区：Q3

影响因子：2.4

年份：2024

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词："Hydrocephalus; Long noncoding RNA growth arrest-specific 5; MicroRNA-325-3p; Chaperonin containing T-complex protein 1, subunit 8; Inflammatory responses; Neurological function"

摘要："Intraventricular hemorrhage results in posthemorrhagic hydrocephalus (PHH). Neonatal hydrocephalus remains a challenging disease due to the high failure rate of all management strategies. We evaluated long noncoding RNA growth arrest-specific 5 (GAS5)-mediated network in neonatal hydrocephalus, providing a new direction for the treatment of hydrocephalus. The PHH model was constructed in neonatal rats after intracerebroventricular injection with GAS5, miR-325-3p, and chaperonin containing T-complex protein 1, subunit 8 (CCT8) plasmids, or oligonucleotides. Next, behavioral tests, measurement of serum inflammation, observation of brain tissue pathology, and calculation of hemoglobin and brain water contents were implemented. GAS5, miR-325-3p, and CCT8 expression, in combination with their interactions, was checked. As the results reported, collagenase infusion induced hydrocephalus, impairing neurological function, enhancing inflammation and neuronal apoptosis, and increasing hemoglobin and brain water contents. GAS5 and CCT8 were up-regulated, while miR-325-3p was down-regulated in hydrocephalic rats. Downregulating GAS5/CCT8 or upregulating miR-325-3p could inhibit inflammatory response and improve neurological function in young hydrocephalic rats. GAS5 promotes CCT8 expression through sponge adsorption of miR-325-3p. GAS5 silencing-mediated protections against hydrocephalus were counteracted by CCT8 overexpression. In summary, GAS5 aggravates neonatal hydrocephalus and inflammatory responses in a way of leasing miR-325-3p-involved regulation of CCT8."

基金机构：National Natural Science Foundation of China

基金资助正文：Not applicable.