Effect of CHRDL1 on angiogenesis and metastasis of colorectal cancer cells via TGF-β/VEGF pathway

作者全名："Li, Junfeng; Jiang, Zhongxiang; He, Jin; Yang, Kun; Chen, Jun; Deng, Qianxi; Li, Xiaoqing; Wu, Fan; Xu, Shuman; Jiang, Zheng"

作者地址："[Li, Junfeng; Jiang, Zhongxiang; He, Jin; Yang, Kun; Chen, Jun; Deng, Qianxi; Li, Xiaoqing; Wu, Fan; Xu, Shuman; Jiang, Zheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Chongqing, Peoples R China; [Jiang, Zheng] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Chongqing 400016, Peoples R China"

通信作者："Jiang, Z (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Chongqing 400016, Peoples R China."

来源：MOLECULAR CARCINOGENESIS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001174473800001

JCR分区：Q2

影响因子：3

年份：2024

卷号：　

期号：　

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文献类型：Article; Early Access

关键词：antiangiogenesis; gene expression; prognostic

摘要："Colorectal cancer (CRC) is a common digestive tract tumor with the third incidence and death in the world. There is still an urgent need for effective therapeutic targets and prognostic markers for CRC. Herein, we report a novel potential target and marker, Chordin like-1 (CHRDL1). The function of CHRDL1 has been reported in gastric cancer, breast cancer, and oral squamous cell carcinoma. However, the biological effect of CHRDL1 in CRC remains unrevealed. Transwell and tube formation experiments were used to determine the biological function of CHRDL1. Western blot and rescue experiments were used to determine the specific mechanisms of CHRDL1. Results showed CHRDL1 is significantly downregulated in CRC cell lines and tissues. In vitro, experiments confirmed that CHRDL1 can inhibit cell growth, migration, invasion, angiogenesis and reverse epithelial-mesenchymal transformation. In vivo, experiments proved that it can inhibit tumor growth and metastasis. Mechanistically, we newly find that CHRDL1 exerts biological functions through the transforming growth factor-beta (TGF-beta)/vascular endothelial growth factor signaling axis in vitro and in vivo. Therefore, we concluded that CHRDL1 reduces the growth, migration, and angiogenesis of CRC cells by downregulating TGF-beta signaling. Our new findings on CHRDL1 may provide a basis for clinical antiangiogenesis therapy and the prognosis of CRC."

基金机构："Experimental Research Center of the First Affiliated Hospital of Chongqing Medical University (Chongqing, China); Chongqing Key Laboratory of Molecular Oncology"

基金资助正文："We would like to thank the Experimental Research Center of the First Affiliated Hospital of Chongqing Medical University (Chongqing, China) and the Chongqing Key Laboratory of Molecular Oncology and Epigenetics (Chongqing, China) for technical guidance."