Anti-Cariogenic Effects of <i>S. cerevisiae</i> and <i>S. boulardii</i> in <i>S. mutans-C. albicans</i> Cross-Kingdom In Vitro Models

作者全名："Yousif, Dina; Wu, Yan; Gonzales, Alexandria Azul; Mathieu, Christa; Zeng, Yan; Sample, Lee; Terando, Sabrina; Li, Ting; Xiao, Jin"

作者地址："[Yousif, Dina; Wu, Yan; Zeng, Yan; Sample, Lee; Li, Ting; Xiao, Jin] Univ Rochester, Med Ctr, Eastman Inst Oral Hlth, Rochester, NY 14642 USA; [Wu, Yan] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Stomatol, Wuhan 430042, Peoples R China; [Gonzales, Alexandria Azul] Univ Rochester, Med Ctr, Sch Med & Dent, Dept Pharmacol & Physiol, Rochester, NY 14642 USA; [Mathieu, Christa] Virginia Commonwealth Univ, VCU Coll Hlth Profess, Richmond, VA 23284 USA; [Terando, Sabrina] Univ Rochester, Sch Arts & Sci, Rochester, NY 14627 USA; [Li, Ting] Chongqing Med Univ, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Xiao, J (通讯作者)，Univ Rochester, Med Ctr, Eastman Inst Oral Hlth, Rochester, NY 14642 USA."

来源：PHARMACEUTICS

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001175138800001

JCR分区：Q1

影响因子：4.9

年份：2024

卷号：16

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：Saccharomyces cerevisiae; Saccharomyces boulardii; Candida albicans; cross-kingdom interaction; pH; dental caries

摘要："Despite the well-documented health benefits of the probiotic Saccharomyces, its application in oral health has not been comprehensively assessed. Dental caries is a transmissible disease initiated by acid production of cariogenic bacteria and yeast, such as Streptococcus mutans and Candida albicans, on tooth enamel and followed by subsequent enamel demineralization. Here, we investigated the effect of two Saccharomyces strains (Saccharomyces boulardii and Saccharomyces cerevisiae) on S. mutans-C. albicans cross-kingdom interactions using a cariogenic planktonic model. Viable cells, pH changes, and gene expression were measured. S. cerevisiae and S. boulardii inhibited the growth of C. albicans in dual- and multi-species conditions at 4, 6, and 20 h. Saccharomyces also inhibited C. albicans hyphal formation. Furthermore, Saccharomyces reduced the acidity of the culture medium, which usually plummeted below pH 5 when S. mutans and C. albicans were present in the model. The presence of Saccharomyces maintained the culture medium above 6 even after overnight incubation, demonstrating a protective potential against dental enamel demineralization. S. boulardii significantly down-regulated S. mutans atpD and eno gene expression. Overall, our results shed light on a new promising candidate, Saccharomyces, for dental caries prevention due to its potential to create a less cariogenic environment marked by a neutral pH and reduced growth of C. albicans."

基金机构：NIH/NIDCR

基金资助正文：No Statement Available