The prognostic value of LAYN in HPV-related head and neck squamous cell carcinoma and its influence on immune cell infiltration

作者全名："Chen, Qingjuan; Chen, Jiankang; Lu, Zuzhuang; Nian, Rui; Li, Wanjun; Yao, Zhongqiang; Mou, Shangdong; Liu, Ying; Cao, Xia; He, Wenjing; Zhu, Chenjing"

作者地址："[Chen, Qingjuan; Yao, Zhongqiang; Mou, Shangdong; Liu, Ying; Cao, Xia] Xi An Jiao Tong Univ, Dept Oncol, Hosp 3201, Hlth Sci Ctr, Hanzhong 723000, Shaanxi, Peoples R China; [Chen, Qingjuan; Chen, Jiankang; Lu, Zuzhuang] Chongqing Med Univ, Dept Oncol, Yongchuan Hosp, Chongqing 40016, Peoples R China; [Nian, Rui; Li, Wanjun] Xi An Jiao Tong Univ, Dept Pathol, Hosp 3201, Hlth Sci Ctr, Hanzhong 723000, Shaanxi, Peoples R China; [He, Wenjing] Xianyang Ctr Hosp, Dept Oncol, Xian 712000, Shaanxi, Peoples R China; [Zhu, Chenjing] Nanjing Med Univ, Dept Radiat Oncol, Jiangsu Canc Hosp, 42 Baiziting, Nanjing 210009, Jiangsu, Peoples R China; [Zhu, Chenjing] Nanjing Med Univ, Jiangsu Inst Canc Res, 42 Baiziting, Nanjing 210009, Jiangsu, Peoples R China; [Zhu, Chenjing] Affiliated Canc Hosp Nanjing Med Univ, 42 Baiziting, Nanjing 210009, Jiangsu, Peoples R China"

通信作者："He, WJ (通讯作者)，Xianyang Ctr Hosp, Dept Oncol, Xian 712000, Shaanxi, Peoples R China.; Zhu, CJ (通讯作者)，Nanjing Med Univ, Dept Radiat Oncol, Jiangsu Canc Hosp, 42 Baiziting, Nanjing 210009, Jiangsu, Peoples R China.; Zhu, CJ (通讯作者)，Nanjing Med Univ, Jiangsu Inst Canc Res, 42 Baiziting, Nanjing 210009, Jiangsu, Peoples R China.; Zhu, CJ (通讯作者)，Affiliated Canc Hosp Nanjing Med Univ, 42 Baiziting, Nanjing 210009, Jiangsu, Peoples R China."

来源：DISCOVER ONCOLOGY

ESI学科分类：　

WOS号：WOS:001175180900001

JCR分区：Q3

影响因子：2.2

年份：2024

卷号：15

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：LAYN; Prognosis; HPV; HNSCC; Immune cell infiltration

摘要："Background HPV-positive head and neck squamous cell carcinoma (HNSCC) exhibits different characteristics from HPV-negative tumors in terms of tumor development, clinical features, treatment response, and prognosis. Layilin (LAYN), which contains homology with C-type lectins, plays a critical role in tumorigenesis and cancer progression. However, the prognostic value of LAYN and the relationship between LAYN and immune infiltration levels in HPV-related HNSCC patients still require a comprehensive understanding. Herein, we aimed to assess the prognostic value of LAYN and to investigate its underlying immunological function in HPV-related HNSCC. Methods Through various bioinformatics methods, we analyzed the data from The Cancer Genome Atlas (TCGA), Tumor Immune Estimation Resource (TIMER) and Gene Expression Profiling Interactive Analysis (GEPIA) databases to explore the potential underlying oncogenic impression of LAYN, including the relevance of LAYN to survival outcomes, clinicopathological factors, immune cell infiltration, and immune marker sets in HPV-related HNSCC. The expression levels of LAYN and HPV were also verified in HNSCC patient tissues. Results LAYN was differentially expressed in a variety of tumors. The expression of LAYN in HNSCC was significantly higher than that in adjacent normal tissues (P < 0.0001), and high expression of LAYN was correlated with poor overall survival (OS) in HNSCC patients (Hazard Ratio (HR) = 1.3, P = 0.035). Moreover, LAYN expression level in HPV-positive HNSCC patients was significantly lower than that in HPV-negative patients, with HPV-positive HNSCC patients displaying a trend of favorable prognosis. In addition, the relationship between LAYN expression and immune infiltration levels in HPV-positive HNSCC group was less tightly correlated than that in HPV-negative HNSCC group, and there was a strong relationship between LAYN expression and markers of M2 macrophage (P < 0.001) and exhausted T cells (P < 0.05) in HPV-negative HNSCC. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis suggested that LAYN potentially influenced tumor progression through HPV infection and other cancer-related pathways. Conclusions LAYN might contribute to tumorigenesis via its positive correlation with immune checkpoint molecules and tumor-associated macrophages (TAMs). Our study might provide a novel prognostic biomarker and latent therapeutic target for the treatment of HPV-related HNSCC."

基金机构：Chongqing Science and Health Joint Medical Research Fund

基金资助正文：No Statement Available