Comparisons of different extraction methods and solvents for saliva samples

作者全名："Fang, Lingli; Zhai, Qiming; Zhang, Hua; Ji, Ping; Chen, Chang; Zhang, Hongmei"

作者地址："[Fang, Lingli; Zhai, Qiming; Ji, Ping] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China; [Zhang, Hongmei] Chongqing Med Univ, Dept Pediat Dent, Stomatol Hosp, Chongqing, Peoples R China; [Chen, Chang] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China; [Zhang, Hua] Chongqing Med Univ, Dept Obstet, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Zhang, HM (通讯作者)，Chongqing Med Univ, Dept Pediat Dent, Stomatol Hosp, Chongqing, Peoples R China.; Chen, C (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China."

来源：METABOLOMICS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001177872400001

JCR分区：Q2

影响因子：3.5

年份：2024

卷号：20

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：Salivary metabolites; Metabolomics; Sample preparation; LC-MS

摘要："IntroductionChanges in the categories and concentrations of salivary metabolites may be closely related to oral, intestinal or systemic diseases. To study salivary metabolites, the first analytical step is to extract them from saliva samples as much as possible, while reducing interferences to a minimum. Frequently used extraction methods are protein precipitation (PPT), liquid-liquid extraction (LLE) and solid-phase extraction (SPE), with various organic solvents. The types and quantities of metabolites extracted with different methods may vary greatly, but few studies have systematically evaluated them.ObjectivesThis study aimed to select the most suitable methods and solvents for the extraction of saliva according to different analytical targets.MethodsAn untargeted metabolomics approach based on liquid chromatography-mass spectrometry was applied to obtain the raw data. The numbers of metabolites, repeatability of the data and intensities of mass spectrometry signals were used as evaluation criteria.ResultsPPT resulted in the highest coverage. Among the PPT solvents, acetonitrile displayed the best repeatability and the highest coverage, while acetone resulted in the best signal intensities for the extracted compounds. LLE with the mixture of chloroform and methanol was the most suitable for the extraction of small hydrophobic compounds.ConclusionPPT with acetonitrile or acetone was recommended for untargeted analysis, while LLE with the mixture of chloroform and methanol was recommended for small hydrophobic compounds."

基金机构：National Natural Science Foundation of China

基金资助正文：We thank the technical support provided by Xia Zhang.