The GDNF-gel/HA-Mg conduit promotes the repair of peripheral nerve defects by regulating PPAR-g/RhoA/ROCK signaling pathway

作者全名："Cai, Yuanqing; Chen, Yi; Zhang, Guangyang; Lin, Yi; Zhang, Jianan; Liang, Jialin; Lv, Leifeng; Wang, Yong; Fang, Xinyu; Dang, Xiaoqian"

作者地址："[Cai, Yuanqing; Zhang, Guangyang; Zhang, Jianan; Liang, Jialin; Lv, Leifeng; Dang, Xiaoqian] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Orthopaed, Xian 710006, Peoples R China; [Chen, Yi; Wang, Yong] Chongqing Univ, Coll Mat Sci & Engn, Natl Engn Res Ctr Magnesium Alloys, Chongqing 400045, Peoples R China; [Chen, Yi] Chongqing Med Univ, Affiliated Hosp 3, Dept Ophthalmol, Chongqing 401120, Peoples R China; [Zhang, Jianan] Xi An Jiao Tong Univ, Zonglian Coll, Xian 710054, Peoples R China; [Fang, Xinyu] Fujian Med Univ, Affiliated Hosp 1, Dept Orthopaed Surg, Fuzhou 350005, Peoples R China"

通信作者："Dang, XQ (通讯作者)，Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Orthopaed, Xian 710006, Peoples R China.; Wang, Y (通讯作者)，Chongqing Univ, Coll Mat Sci & Engn, Natl Engn Res Ctr Magnesium Alloys, Chongqing 400045, Peoples R China.; Fang, XY (通讯作者)，Fujian Med Univ, Affiliated Hosp 1, Dept Orthopaed Surg, Fuzhou 350005, Peoples R China."

来源：ISCIENCE

ESI学科分类：　

WOS号：WOS:001178712600001

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：27

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Magnesium (Mg) -based conduits have gained more attention in repairing peripheral nerve defects. However, they are limited due to poor corrosion resistance and rapid degradation rate. To tackle this issue, glial cell line -derived neurotrophic factor (GDNF)- Gelatin methacryloyl (Gel)/hydroxylapatite (HA) -Mg nerve conduit was developed and implanted in sciatic nerve defect model in Sprague-Dawley (SD) rats. The sciatic functional index measurement showed that the GDNF-Gel/HA-Mg nerve conduit effectively promoted the recovery of sciatic nerve function. The pathological examination results showed that there were more regenerated nerve tissues in GDNF-Gel/HA-Mg group, with a higher number of regenerating axons, and the thickness of the myelin sheath was significantly larger than that of control group (NC group). Immunofluorescence results revealed that the GDNF-Gel/HA-Mg conduit significantly promoted the expression of genes associated with nerve repair. RNA-seq and molecular test results indicated that GDNF-Gel/HA-Mg might be involved in the repair of peripheral nerve defects by regulating PPAR-g/ RhoA/ROCK signaling pathway. Biological sciences; Neuroscience; Molecular neuroscience; Techniques in neuroscience"

基金机构："National Natural Science Foundation of China [82171370]; Key Research and Development Program of the Shaanxi Province [2019SF-113, 2022SF-192]; Foreign Cooperation Project of Science and Technology, Fujian Province [2021I0012]"

基金资助正文："The present work was supported by National Natural Science Foundation of China (82171370) ; Key Research and Development Program of the Shaanxi Province (2019SF-113; 2022SF-192) ; Foreign Cooperation Project of Science and Technology, Fujian Province (2021I0012) ."