Molecular insights into the proteomic composition of porcine treated dentin matrix

作者全名："Zhang, Xiya; Zhou, Sha; Zhan, Yuzhen; Mei, Ziyi; Qian, Aizhuo; Yuan, Yu; Zhang, Xiaonan; Fu, Tiwei; Ma, Shiyong; Li, Jie"

作者地址："[Zhang, Xiya; Zhou, Sha; Zhan, Yuzhen; Mei, Ziyi; Qian, Aizhuo; Yuan, Yu; Zhang, Xiaonan; Fu, Tiwei; Li, Jie] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Zhang, Xiya; Zhou, Sha; Zhan, Yuzhen; Mei, Ziyi; Qian, Aizhuo; Yuan, Yu; Zhang, Xiaonan; Fu, Tiwei; Li, Jie] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Zhang, Xiya; Zhou, Sha; Zhan, Yuzhen; Mei, Ziyi; Qian, Aizhuo; Yuan, Yu; Zhang, Xiaonan; Fu, Tiwei; Li, Jie] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Ma, Shiyong] Chongqing Med Univ, Inst Life Sci, Basic Med Res & Innovat Ctr Novel Target & Therape, Minist Educ, Chongqing, Peoples R China; [Li, Jie] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China; [Ma, Shiyong] Chongqing Med Univ, Inst Life Sci, Basic Med Res & Innovat Ctr Novel Target & Therape, Minist Educ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Li, J (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China.; Ma, SY (通讯作者)，Chongqing Med Univ, Inst Life Sci, Basic Med Res & Innovat Ctr Novel Target & Therape, Minist Educ, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：MATERIALS TODAY BIO

ESI学科分类：　

WOS号：WOS:001179536500001

JCR分区：Q1

影响因子：8.7

年份：2024

卷号：25

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Extracellular matrix; Treated dentin matrix; Dentin-pulp regeneration; Proteomics; Stem cells from human exfoliated deciduous; teeth

摘要："Background: Human -treated dentin matrix (hTDM) has recently been studied as a natural extracellular matrixbased biomaterial for dentin pulp regeneration. However, porcine -treated dentin matrix (pTDM) is a potential alternative scaffold due to limited availability. However, there is a dearth of information regarding the protein composition and underlying molecular mechanisms of pTDM. Methods: hTDM and pTDM were fabricated using human and porcine teeth, respectively, and their morphological characteristics were examined using scanning electron microscopy. Stem cells derived from human exfoliated deciduous teeth (SHEDs) were isolated and characterized using flow cytometry and multilineage differentiation assays. SHEDs were cultured in three-dimensional environments with hTDM, pTDM, or biphasic hydroxyapatite/tricalcium phosphate. The expression of odontogenesis markers in SHEDs were assessed using real-time polymerase chain reaction and immunochemical staining. Subsequently, SHEDs/TDM and SHEDs/HA/ TCP complexes were transplanted subcutaneously into nude mice. The protein composition of pTDM was analyzed using proteomics and compared to previously published data on hTDM. Results: pTDM and hTDM elicited comparable upregulation of odontogenesis-related genes and proteins in SHEDs. Furthermore, both demonstrated the capacity to stimulate root -related tissue regeneration in vivo. Proteomic analysis revealed the presence of 278 protein groups in pTDM, with collagens being the most abundant. Additionally, pTDM and hTDM shared 58 identical proteins, which may contribute to their similar abilities to induce odontogenesis. Conclusions: Both hTDM and pTDM exhibit comparable capabilities in inducing odontogenesis, potentially owing to their distinctive bioactive molecular networks."

基金机构："National Natural Science Founda-tion of China [82071072]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0179]; Scientific and Young and Middle-aged Senior Medical Talents studio of Chongqing [ZQNYXGDRCGZS2019004]"

基金资助正文："<B>Funding</B> This study was supported by the National Natural Science Founda-tion of China [Grant No. 82071072] , Program for Youth Innovation in Future Medicine, Chongqing Medical University [Grant No. W0179] , and Scientific and Young and Middle-aged Senior Medical Talents studio of Chongqing [Grant No. ZQNYXGDRCGZS2019004] ."