A hydrogel derived from skin secretion of<i> Andrias</i><i> davidianus</i> to facilitate bone regeneration

作者全名："Zhang, Qi; Feng, Xing; Peng, Siyu; Li, Lan; Xiang, Yangfan; Feng, Tengchao; Zhang, Ximu; Song, Jinlin"

作者地址："[Zhang, Qi; Feng, Xing; Peng, Siyu; Li, Lan; Xiang, Yangfan; Feng, Tengchao; Zhang, Ximu; Song, Jinlin] Chongqing Med Univ, Stomatol Hosp, Chongqing 401174, Peoples R China; [Zhang, Qi; Feng, Xing; Peng, Siyu; Li, Lan; Xiang, Yangfan; Feng, Tengchao; Zhang, Ximu; Song, Jinlin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401174, Peoples R China; [Zhang, Qi; Feng, Xing; Peng, Siyu; Li, Lan; Xiang, Yangfan; Feng, Tengchao; Zhang, Ximu; Song, Jinlin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401174, Peoples R China"

通信作者："Zhang, XM; Song, JL (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing 401174, Peoples R China."

来源：COMPOSITES PART B-ENGINEERING

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001180605300001

JCR分区：Q1

影响因子：12.7

年份：2024

卷号：274

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Andrias davidianus; Bone regeneration; Angiogenesis; Hydrogel; Visible light

摘要："Bone regeneration relies heavily on balancing osteogenesis and osteoclastogenesis. Meeting the challenge of creating bone repair scaffolds with dual functionalities has long been elusive in bone tissue engineering. This study addresses these challenges through the utilization of a unique hydrogel that capitalizes on the dynamics of disulfide bonding to convert the skin secretion of Andrias davidianus (SSAD) into hydrolysate. This hydrolysate serves as the foundation for the construction of a novel SSAD protein hydrogel for bone regeneration, achieved through a photo-redox reaction under visible light crosslinking. The innovative SSAD protein hydrogel not only exhibits exceptional biocompatibility but also significantly enhances bone mineralization and angiogenesis in vitro. RNA sequencing delves into the underlying mechanisms governing the regulation of osteogenesis and osteoclastogenesis by the hydrogel. In vivo experiments conclusively demonstrate the potential of the SSAD protein hydrogel. It expedites vascularization within the defect area and synergistically facilitates the repair of bone defects. Furthermore, the SSAD hydrogel exhibits notable antimicrobial properties, effectively reducing the risk of implant-related infections in vivo. In summary, this research introduces a valuable and promising method for fabricating protein biomaterials that contribute to bone repair by promoting both bone and blood vessel growth while simultaneously inhibiting osteoclast formation."

基金机构：National Natural Science Foundation of China [32070826]; National Key R & D of Program of China [2022YFC2504200]; Chongqing Science and Health Joint Medical project [2020GDRC017]; CQMU Program for Youth Innovation in Future Medicine [W0075]; Senior Medical Talents Program of Chongqing for Young and Middle-aged

基金资助正文："This work was financed by the National Natural Science Foundation of China (Grant No. U22A20314) , National Key R & D of Program of China (Grant NO. 2022YFC2504200) , the National Natural Science Foundation of China (32070826) , Chongqing Science and Health Joint Medical project (2020GDRC017) , CQMU Program for Youth Innovation in Future Medicine (W0075) , Senior Medical Talents Program of Chongqing for Young and Middle-aged."