A single cell atlas of circulating immune cells involved in diabetic retinopathy

作者全名："Liao, Dan; Fan, Wei; Li, Na; Li, Ruonan; Wang, Xiaotang; Liu, Jiangyi; Wang, Hong; Hou, Shengping"

作者地址："[Liao, Dan; Fan, Wei; Li, Ruonan; Wang, Xiaotang; Liu, Jiangyi; Hou, Shengping] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Eye Inst, Chongqing Key Lab Ophthalmol, Chongqing 400016, Peoples R China; [Liao, Dan] North Sichuan Med Coll, Affiliated Hosp, Nanchong 637000, Sichuan, Peoples R China; [Li, Na] Chongqing Med Univ, Sch Basic Med Sci, Chongqing 400016, Peoples R China; [Wang, Hong] Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Ophthalmol & Visual Sci Key Lab, Beijing 100730, Peoples R China; [Hou, Shengping] Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Ophthalmol, Beijing Tongren Eye Ctr,Beijing Ophthalmol & Visua, Beijing 100730, Peoples R China"

通信作者："Hou, SP (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Chongqing Eye Inst, Chongqing Key Lab Ophthalmol, Chongqing 400016, Peoples R China.; Hou, SP (通讯作者)，Capital Med Univ, Beijing Tongren Hosp, Beijing Inst Ophthalmol, Beijing Tongren Eye Ctr,Beijing Ophthalmol & Visua, Beijing 100730, Peoples R China."

来源：ISCIENCE

ESI学科分类：　

WOS号：WOS:001181384300001

JCR分区：Q1

影响因子：4.6

年份：2024

卷号：27

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："This study focused on examining the exact role of circulating immune cells in the development of diabetic retinopathy (DR). In vitro co -culture experiments showed that peripheral blood mononuclear cells (PBMCs) from patients with type 1 DR crucially modulated the biological functions of human retinal microvascular endothelial cells (HRMECs), consequently disrupting their normal functionality. Single -cell RNA sequencing (scRNA-seq) study revealed unique differentially expressed genes and pathways in circulating immune cells among healthy controls, non -diabetic retinopathy (NDR) patients, and DR patients. Of significance was the observed upregulation of JUND in each subset of PBMCs in patients with type 1 DR. Further studies showed that downregulating JUND in DR patient -derived PBMCs led to the amelioration of HRMEC dysfunction. These findings highlighted the notable alterations in the transcriptomic patterns of circulating immune cells in type 1 DR patients and underscored the significance of JUND as a key factor for PBMCs in participating in the pathogenesis of DR."

基金机构："Natural Science Foundation Project of China [82070951, 82271078, 82371045]; Beijing Natural Science Foundation [7212016]"

基金资助正文："This work was supported by Natural Science Foundation Project of China (82070951, 82271078, and 82371045) , and Beijing Natural Science Foundation (7212016) ."