Polyphenol Fraction of<i> Cyclocarya</i><i> Paliurus</i> Leaves Modulates Intestinal Microbiota and Alleviates Type 2 Diabetes Mellitus in Mice

作者全名："Huang, Haifeng; Guo, Wenjia; Ning, Ziwan; Xue, Deao; Xiao, Haitao; Yang, Junqing"

作者地址："[Huang, Haifeng; Yang, Junqing] Chongqing Med Univ, Dept Pharmacol, Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China; [Guo, Wenjia] Chongqing Med Univ, Dept Pharm, Women & Childrens Hosp, Chongqing 401147, Peoples R China; [Guo, Wenjia] Chongqing Hlth Ctr Women & Children, Dept Pharm, Chongqing 400021, Peoples R China; [Ning, Ziwan] Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong 999077, Peoples R China; [Xue, Deao; Xiao, Haitao] Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Shenzhen 518060, Guangdong, Peoples R China"

通信作者："Yang, JQ (通讯作者)，Chongqing Med Univ, Dept Pharmacol, Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China.; Xiao, HT (通讯作者)，Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Shenzhen 518060, Guangdong, Peoples R China."

来源：JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001182330300001

JCR分区：Q4

影响因子：0.8

年份：2024

卷号：38

期号：3

开始页：2125

结束页：2138

文献类型：Article

关键词：type 2 diabetes mellitus; Cyclocarya paliurus; insulin resistance; intestinal microbiota

摘要："Background: Type 2 diabetes mellitus has become a serious social concern, and diet-based therapies, such as those involving the Cyclocarya paliurus leave tea are gaining attention for preventing and alleviating diabetes mellitus. The objective of the present study was to determine the active components of this tea and understand the associated mechanism. Methods: The active fraction of C. paliurus was traced using the Cell Counting Kit -8 in response to streptozotocin-induced cell damage, and its components were identified using ultra-performance liquid chromatography coupled with quadrupole time-offlight mass spectrometry. Flow cytometry and western blotting were performed to examine the effects of the active fraction on streptozotocin-induced pancreatic beta cell damage. A high-fat diet combined with streptozotocin-induced animal model was used to identify the effects of this extract against type 2 diabetes mellitus following 8 weeks of gavage administration. Results: The polyphenol fraction of C. paliurus protected against streptozotocin-induced cell damage and up-regulated cleaved caspase-3 and the Bax/Bcl-2 ratio in pancreatic beta cells (p < 0.05). Moreover, this administration of the extract effectively reduced body weight, blood glucose level, and increased insulin sensitivity (p < 0.05). The treatment also alerted the abundance of gut microbiota, particularly by upregulating the abundance of Akkermansia and decreasing pathogenic bacteria genus Helicobacter (p < 0.05). Conclusions: Our findings suggested that the polyphenol fraction of C. paliurus could be a potential candidate for gut microbiota modulation as a dietary component for the prevention or management of type 2 diabetes mellitus."

基金机构：Chongqing Science and Technology Commission Fund [cstc2015zdcy-ztzx120003]; Foundations of Shen-zhen Science and Technology Innovation Committee [JCYJ20210324093810026]

基金资助正文：This study was financially supported by Chongqing Science and Technology Commission Fund (cstc2015zdcy-ztzx120003) and Foundations of Shen-zhen Science and Technology Innovation Committee (JCYJ20210324093810026) .