Accurate and sensitive low-density lipoprotein (LDL) detection based on the proximity ligation assisted rolling circle amplification (RCA)

作者全名："Zhang, Xingyu; Li, Jie; Yang, Mei; Huang, Hong; Wang, Hao; Zhang, Hongmin"

作者地址："[Zhang, Xingyu; Yang, Mei; Zhang, Hongmin] Chongqing Liang Jiang New Area, Dept Endocrinol, Peoples Hosp, 199 Renxing Rd, Chongqing 401121, Peoples R China; [Li, Jie] Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Key Lab Lab Med Diagnost,Minist Educ, Chongqing 400016, Peoples R China; [Wang, Hao] Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, Chongqing 400016, Peoples R China; [Huang, Hong] Peoples Hosp, Dept Clin Lab, Chongqing Liang Jiang New Area, Chongqing 401121, Peoples R China"

通信作者："Zhang, HM (通讯作者)，Chongqing Liang Jiang New Area, Dept Endocrinol, Peoples Hosp, 199 Renxing Rd, Chongqing 401121, Peoples R China."

来源：ANALYTICAL METHODS

ESI学科分类：CHEMISTRY

WOS号：WOS:001184672500001

JCR分区：Q1

影响因子：2.7

年份：2024

卷号：16

期号：13

开始页：1894

结束页：1900

文献类型：Article

关键词：　

摘要："Metabolic-associated fatty liver disease (MAFLD) is one of the leading causes of mortality from chronic diseases worldwide, and it is strongly linked to dyslipidemia. Dyslipidemia typically presents as an elevated concentration of low density lipoprotein (LDL). Hence, accurate quantification of LDL particles is crucial for predicting the risks of cardiovascular illnesses. Nevertheless, conventional techniques can merely provide indirect measurements of LDL particle concentrations through the detection of cholesterol or proteins within LDL particles, and they often require significant effort and time. Therefore, an accurate and effective method for identifying intact LDL particles is highly desired. We have devised a method that allows for the measurement of LDL concentration without the need for isolation. This method relies on proximity ligation rolling circle amplification (RCA). This technique enables the direct and precise measurement of the concentration of ""actual"" LDL particles, rather than measuring the cholesterol content inside LDL. It has a detection limit of 7.3 mu g dL-1, which also meets the requirements for analyzing lipoproteins in clinical samples. Hence, this platform exhibits immense potential in clinical applications and health management. Accurate and sensitive low-density lipoprotein (LDL) detection."

基金机构：Natural Science Foundation of Chongqing Municipality [2022MSXM133]; Chongqing Science and Health Joint Medical Research Project [CSTB2022NSCQ-MSX1522]; Natural Science Foundation of Chongqing; First batch of key Disciplines On Public Health in Chongqing

基金资助正文：This work was partly supported by the grants from Chongqing Science and Health Joint Medical Research Project (Project No. 2022MSXM133) and Natural Science Foundation of Chongqing (Project No. CSTB2022NSCQ-MSX1522). The First batch of key Disciplines On Public Health in Chongqing.