RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression
作者全名："Ren, Junwu; Huang, Bo; Li, Wei; Wang, Yongquan; Pan, Xiaojuan; Ma, Qiang; Liu, Yuying; Wang, Xiaolin; Liang, Ce; Zhang, Yuying; Wang, Shimin; Yang, Feifei; Li, Haiping; Ning, Hao; Jiang, Yan; Qin, Changhong; Ran, Ai; Xiao, Bin"
作者地址："[Ren, Junwu; Huang, Bo; Pan, Xiaojuan; Ma, Qiang; Liu, Yuying; Wang, Xiaolin; Liang, Ce; Zhang, Yuying; Wang, Shimin; Yang, Feifei; Li, Haiping; Ning, Hao; Jiang, Yan; Qin, Changhong; Ran, Ai; Xiao, Bin] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Huang, Bo] Zunyi Med Univ, Key Lab Basic Pharmacol, Minist Educ & Joint Int Res Lab Ethnomed, Zunyi 563000, Peoples R China; [Li, Wei] Chongqing Univ, Canc Hosp, Dept Pharm, Chongqing 400030, Peoples R China; [Wang, Yongquan] Army Med Univ, Southwest Hosp, Dept Urol, Chongqing 400038, Peoples R China"
通信作者："Xiao, B (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China."
来源：TRANSLATIONAL ONCOLOGY
ESI学科分类：CLINICAL MEDICINE
WOS号：WOS:001184947700001
JCR分区：Q1
影响因子：4.5
年份：2024
卷号：42
期号：
开始页：
结束页：
文献类型：Article
关键词：Clear cell renal cell carcinoma; IGF2BP2; CKB; mRNA stability; RNA binding protein
摘要："Clear cell renal cell carcinoma (ccRCC) is the most prevalent kidney cancer, with a highly aggressive phenotype and poor prognosis. RNA binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation and have been implicated in tumorigenesis. RBPs have the potential to become a new therapeutic target for ccRCC. In this study, we screened and validated that insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) as an RBP, was down-regulated in ccRCC tissues and cell lines. Functionally, we verified that IGF2BP2 significantly suppressed the migration and invasion ability of ccRCC in vitro and in vivo. Mechanistically, RIP-seq and actinomycin D experiments results showed that IGF2BP2 enhanced the expression of Creatine Kinase B (CKB) by binding to CKB mRNA and enhancing its mRNA stability. Thus, IGF2BP2 inhibited ccRCC metastasis through enhancing the expression of CKB. Taken together, these finding suggests that IGF2BP2 is a novel metastasis suppressor of ccRCC and may serve as a potential therapeutic target."
基金机构："National Natural Science Foundation of China [82373001, 82073254, 81872392]; Chongqing Talents-Exceptional Young Talents Project [CQYC202005044, CSTC2021YCJH-BGZXM0094]; Chongqing Natural Science Foundation Innovation and Development Joint Fund [CSTB2022NSCQ-LZX0043]; Science and Technology Research Project of Chongqing Municipal Education Commission [KJZD-K202100405]; Future Medical Youth Innovation Team Project of Chongqing Medical University [W0042]; Graduate Tutor Team Construction Project of Chongqing [CQMUDSTD202210]; Top Graduate Talent Cultivation Program of Chongqing Medical University [BJRC202105]; Natural Science Foundation of Chongqing [CSTC2020JCYJ-MSXMX0337, CSTC2022JXJL120012]"
基金资助正文："This study was supported by the National Natural Science Foundation of China (82373001, 82073254, 81872392) , Chongqing Talents-Exceptional Young Talents Project (CQYC202005044, CSTC2021YCJH-BGZXM0094) , Chongqing Natural Science Foundation Innovation and Development Joint Fund (CSTB2022NSCQ-LZX0043) , Science and Technology Research Project of Chongqing Municipal Education Commission (KJZD-K202100405) , Future Medical Youth Innovation Team Project of Chongqing Medical University (W0042) , Graduate Tutor Team Construction Project of Chongqing (CQMUDSTD202210) , Top Graduate Talent Cultivation Program of Chongqing Medical University (BJRC202105) , Natural Science Foundation of Chongqing (CSTC2020JCYJ-MSXMX0337, CSTC2022JXJL120012) ."
