Diagnosis of pulmonary S<i>cedosporium apiospermum</i> infection from bronchoalveolar lavage fluid by metagenomic next-generation sequencing in an immunocompetent female patient with normal lung structure: a case report and literature review

作者全名："Han, Jingru; Liang, Lifang; Li, Qingshu; Deng, Ruihang; Liu, Chenyang; Wu, Xuekai; Zhang, Yuxin; Zhang, Ruowen; Dai, Haiyun"

作者地址："[Han, Jingru] Chongqing Med Univ, Affiliated Hosp 1, Dept Oncol, 1 Youyi Rd, Chongqing 400010, Peoples R China; [Liang, Lifang; Deng, Ruihang; Wu, Xuekai; Zhang, Yuxin; Zhang, Ruowen] Chongqing Med Univ, Coll Clin Med 1, Chongqing 400016, Peoples R China; [Li, Qingshu] Chongqing Med Univ, Sch Basic Med, Dept Pathol, 1 Med Coll Rd, Chongqing 400016, Peoples R China; [Liu, Chenyang] Chongqing Med Univ, Coll Clin Med 2, Chongqing 400016, Peoples R China; [Dai, Haiyun] Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, 1 Youyi Rd, Chongqing 400010, Peoples R China"

通信作者："Dai, HY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Resp & Crit Care Med, 1 Youyi Rd, Chongqing 400010, Peoples R China."

来源：BMC INFECTIOUS DISEASES

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001185073400003

JCR分区：Q2

影响因子：3.4

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Metagenomic next-generation sequencing; Pulmonary infection; Scedosporium Apiospermum

摘要："Background Scedosporium apiospermum (S. apiospermum) belongs to the asexual form of Pseudallescheria boydii and is widely distributed in various environments. S. apiospermum is the most common cause of pulmonary infection; however, invasive diseases are usually limited to patients with immunodeficiency. Case presentation A 54-year-old Chinese non-smoker female patient with normal lung structure and function was diagnosed with pulmonary S. apiospermum infection by metagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage fluid (BALF). The patient was admitted to the hospital after experiencing intermittent right chest pain for 8 months. Chest computed tomography revealed a thick-walled cavity in the upper lobe of the right lung with mild soft tissue enhancement. S. apiospermum was detected by the mNGS of BALF, and DNA sequencing reads were 426. Following treatment with voriconazole (300 mg q12h d1; 200 mg q12h d2-d20), there was no improvement in chest imaging, and a thoracoscopic right upper lobectomy was performed. Postoperative pathological results observed silver staining and PAS-positive oval spores in the alveolar septum, bronchiolar wall, and alveolar cavity, and fungal infection was considered. The patient's symptoms improved; the patient continued voriconazole for 2 months after surgery. No signs of radiological progression or recurrence were observed at the 10-month postoperative follow-up. Conclusion This case report indicates that S. apiospermum infection can occur in immunocompetent individuals and that the mNGS of BALF can assist in its diagnosis and treatment. Additionally, the combined therapy of antifungal drugs and surgery exhibits a potent effect on the disease."

基金机构：　

基金资助正文：