RAMP2-AS1 stabilized RAPM2 mRNA through TIA1 to inhibit the progression of nonsmall cell lung cancer

作者全名："Wang, Siqin; Xu, Ce; Shan, Yuanyuan; Zhang, Yi"

作者地址："[Wang, Siqin] Chongqing Med Univ, Affiliated Hosp Chengdu 2, Chengdu Clin Coll, Peoples Hosp Chengdu 3,Emergency Dept,Affiliated, Chengdu 610031, Sichuan, Peoples R China; [Xu, Ce; Zhang, Yi] Jimin Hosp, Dept Oncol, Shanghai 200052, Peoples R China; [Shan, Yuanyuan] Hangzhou Mushi Biotechnol Co Ltd Hangzhou, Hangzhou 310000, Peoples R China"

通信作者："Zhang, Y (通讯作者)，Jimin Hosp, Dept Oncol, Shanghai 200052, Peoples R China.; Shan, YY (通讯作者)，Hangzhou Mushi Biotechnol Co Ltd Hangzhou, Hangzhou 310000, Peoples R China."

来源：CELLULAR AND MOLECULAR BIOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001185714600002

JCR分区：Q4

影响因子：1.5

年份：2023

卷号：69

期号：14

开始页：9

结束页：14

文献类型：Article

关键词：Non-small cell lung cancer; RAMP2; RAMP2-AS1; TIA1

摘要："As the most common subtype of lung cancer, non-small cell lung cancer (NSCLC)is responsible for a large proportion of global cancer-caused deaths. The implication of long non-coding RNAs (lncRNAs) as tumorsuppressor or carcinogenic genes in NSCLC has been widely documented. Our study sought to investigate the performance of lncRNA RAMP2 antisense RNA1 (RAMP2-AS1) in NSCLC. GEPIA bioinformatics tool and RT-qPCR were applied for assessing the expression of RAMP2-AS1 and its neighboring gene receptor activity-modifying protein 2 (RAMP2) in NSCLC. Functional assays including CCK-8 assay, colony formation assay as well as caspase-3 activity analysis and Transwell invasion assays were applied for detecting the biological phenotypes of NSCLC cells. Interaction among RAMP2-AS1, RAMP2 and T-cell intracellular antigen 1cytotoxic granule associated RNA binding protein (TIA1) was evaluated by RNA immunoprecipitation and pulldown assays. We found that RAMP2-AS1 and RAMP2 were downregulated in NSCLC. Overexpression of RAMP2-AS1 hampered proliferation and invasion, whereas induced apoptosis of NSCLC cells. Mechanistically, RAMP2-AS1 interacted with TIA1 to stabilize the mRNA of RAMP2. In conclusion, we first uncovered that RAMP2-AS1 stabilized RAPM2 mRNA through TIA1 to inhibit the progression of NSCLC, providing new insight to improve the treatment efficacy of NSCLC."

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