Impact of heterogeneous nuclear ribonucleoprotein A/B subtype overexpression on the expression of cancer stem cell markers CD133 and CD44 and cellular proliferation capacity

作者全名："Wang, Meng; Wang, Xian; Cui, Yaping; Wen, Xing"

作者地址："[Wang, Meng; Cui, Yaping; Wen, Xing] Southwest Jiaotong Univ, Ctr Gastrointestinal & Minimally Invas Surg, Peoples Hosp Chengdu 3, Dept Gen Surg,Affiliated Hosp, Chengdu 610031, Peoples R China; [Wang, Xian] Chongqing Med Univ, Affiliated Hosp Chengdu 2, Chengdu 610031, Peoples R China; [Wang, Xian] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Dept Anorectal, Affiliated Hosp, Chengdu 610031, Peoples R China; [Wang, Xian] Chongqing Med Univ, Affiliated Hosp Chengdu 2, Chengdu 610031, Peoples R China"

通信作者："Wen, X (通讯作者)，Southwest Jiaotong Univ, Ctr Gastrointestinal & Minimally Invas Surg, Peoples Hosp Chengdu 3, Dept Gen Surg,Affiliated Hosp, Chengdu 610031, Peoples R China."

来源：CELLULAR AND MOLECULAR BIOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001185714600016

JCR分区：Q4

影响因子：1.5

年份：2023

卷号：69

期号：14

开始页：101

结束页：108

文献类型：Article

关键词：CD133; CD44; Cell proliferation; HnRNPAB; Tumor cell stem cells

摘要："The occurrence and progression of intestinal cancer are complex, multifactorial processes in which tumor stem cells are believed to play a crucial role. An in-depth understanding of their molecular mechanisms holds imperative clinical significance for improving intestinal cancer treatment. HT-29 and HCT116intestinal cancer cell lines were utilized as research models. The experimental group (group E) and control group (group C) were established by transfecting the hnRNPA1 subtype and empty vector, respectively. The expression (EP) levels of hnRNPA1 and changes in Wnt/ss-catenin signaling-related markers were evaluated using techniques such as RNA extraction, reverse transcription reactions, real-time quantitative PCR (RT qPCR), and protein extraction. The EP of tumor stem cell markers CD133 and CD44 was assessed using immunohistochemistry. Additionally, cell invasion assays, scratch assays, and cell counting kit-8 (CCK-8) proliferation assays were conducted. Furthermore, a mouse tumor model was established to observe the growth of tumors in both groups. overexpression (OP) of hnRNPA1 in group E greatly increased the protein EP levels of ss-catenin, Axin2, and Cyclin D1 and exhibited a higher positivity rate for CD133 and CD44. The invasive, migratory, and proliferative abilities of cells in group E were notably enhanced, and tumor growth was observably faster versus group C. OP of hnRNPA1 was closely associated with the activation of Wnt/ss-catenin signaling and promoted the proliferation and invasive capacity of tumor cells. hnRNPA1 played an imperative role in intestinal cancer stem cells."

基金机构：Science and Technology Project of the Health Planning Committee of Chengdu City [2021408]

基金资助正文：The research is supported by: This study was funded by The Science and Technology Project of the Health Planning Committee of Chengdu City (NO. 2021408).