Intact lung tissue and bronchoalveolar lavage fluid are both suitable for the evaluation of murine lung microbiome in acute lung injury

作者全名："Zheng, Lijun; Liu, Chengjun; Wang, Hongjing; Zhang, Jun; Mao, Lejiao; Dong, Xiaomei; Hu, Siyao; Li, Na; Pi, Dandan; Qiu, Jingfu; Xu, Feng; Chen, Chengzhi; Zou, Zhen"

作者地址："[Zheng, Lijun; Zhang, Jun; Mao, Lejiao; Dong, Xiaomei; Hu, Siyao; Li, Na; Zou, Zhen] Chongqing Med Univ, Inst Life Sci, Mol Biol Lab Resp Dis, Chongqing 400016, Peoples R China; [Liu, Chengjun; Pi, Dandan; Xu, Feng] Chongqing Med Univ, Childrens Hosp, Dept Pediat, Intens Care Unit, Chongqing 400014, Peoples R China; [Wang, Hongjing; Qiu, Jingfu] Chongqing Med Univ, Sch Publ Hlth, Dept Hlth Lab Technol, Chongqing 400016, Peoples R China; [Chen, Chengzhi] Chongqing Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, Chongqing 400016, Peoples R China; [Zhang, Jun; Chen, Chengzhi; Zou, Zhen] Sch Publ Hlth, Res Ctr Environm & Human Hlth, Chongqing 400016, Peoples R China"

通信作者："Zou, Z (通讯作者)，Chongqing Med Univ, Inst Life Sci, Mol Biol Lab Resp Dis, Chongqing 400016, Peoples R China.; Zou, Z (通讯作者)，Sch Publ Hlth, Res Ctr Environm & Human Hlth, Chongqing 400016, Peoples R China."

来源：MICROBIOME

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001186181000002

JCR分区：Q1

影响因子：13.8

年份：2024

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Lung microbiome; Acute lung injury; 16S rRNA; Actinobacteriota

摘要："BackgroundAccumulating clinical evidence suggests that lung microbiome is closely linked to the progression of pulmonary diseases; however, it is still controversial which specimen type is preferred for the evaluation of lung microbiome.Methods and resultsTo address this issue, we established a classical acute lung injury (ALI) mice model by intratracheal instillation of lipopolysaccharides (LPS). We found that the bacterial DNA obtained from the bronchoalveolar lavage fluid (BALF), intact lung tissue [Lung(i)], lung tissue after perfused [Lung(p)], and feces of one mouse were enough for 16S rRNA sequencing, except the BALF of mice treated with phosphate buffer saline (PBS), which might be due to the biomass of lung microbiome in the BALF were upregulated in the mice treated with LPS. Although the alpha diversity among the three specimens from lungs had minimal differences, Lung(p) had higher sample-to-sample variation compared with BALF and Lung(i). Consistently, PCoA analysis at phylum level indicated that BALF was similar to Lung(i), but not Lung(p), in the lungs of mice treated with LPS, suggesting that BALF and Lung(i) were suitable for the evaluation of lung microbiome in ALI. Importantly, Actinobacteria and Firmicutes were identified as the mostly changed phyla in the lungs and might be important factors involved in the gut-lung axis in ALI mice. Moreover, Actinobacteria and Proteobacteria might play indicative roles in the severity of lung injury.ConclusionThis study shows both Lung(i) and BALF are suitable for the evaluation of murine lung microbiome in ALI, and several bacterial phyla, such as Actinobacteria, may serve as potential biomarkers for the severity of ALI.3ejvnAMUzhChvYv4uP_mb-Video AbstractConclusionThis study shows both Lung(i) and BALF are suitable for the evaluation of murine lung microbiome in ALI, and several bacterial phyla, such as Actinobacteria, may serve as potential biomarkers for the severity of ALI.3ejvnAMUzhChvYv4uP_mb-Video Abstract"

基金机构：Chongqing Talents: Exceptional Young Talents Project

基金资助正文：We thank the efforts of all the members from MBLRD Lab.