Redox proteomics of PANC-1 cells reveals the significance of HIF-1 signaling protein oxidation in pancreatic ductal adenocarcinoma pathogenesis

作者全名："Tan, Chaochao; Chen, Lichun; Guan, Xiaoyu; Huang, Wenyi; Feng, Yinhong; Li, Ziyi; Wu, Ling; Huang, Xiangping; Ouyang, Qianhui; Liu, Sixiang; Huang, Ying; Hu, Jiliang"

作者地址："[Tan, Chaochao; Wu, Ling; Huang, Xiangping; Ouyang, Qianhui] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Clin Lab, Affiliated Hosp 1, Changsha 410005, Peoples R China; [Tan, Chaochao] Hunan Normal Univ, Hunan Prov Peoples Hosp, Tumor Immun Res Ctr Hunan Prov Geriatr Inst, Affiliated Hosp 1, Changsha 410005, Peoples R China; [Chen, Lichun; Guan, Xiaoyu; Huang, Wenyi; Feng, Yinhong; Li, Ziyi; Hu, Jiliang] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China; [Liu, Sixiang; Huang, Ying] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Emergency, Affiliated Hosp 1, Changsha 410006, Hunan, Peoples R China; [Hu, Jiliang] Chongqing Med Univ, Inst Life Sci, Chongqing 400032, Peoples R China"

通信作者："Hu, JL (通讯作者)，Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China.; Huang, Y (通讯作者)，Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Emergency, Affiliated Hosp 1, Changsha 410006, Hunan, Peoples R China.; Hu, JL (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing 400032, Peoples R China."

来源：JOURNAL OF TRANSLATIONAL MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001186513200004

JCR分区：Q1

影响因子：6.1

年份：2024

卷号：22

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Protein cysteine oxidation; iodoTMT; Redox proteomics; Pancreatic ductal adenocarcinoma; PANC-1

摘要："BackgroundProtein cysteine oxidation is substantially involved in various biological and pathogenic processes, but its implications in pancreatic cancer development remains poorly understood.Methods and resultsIn this study, we performed a global characterization of protein oxidation targets in PDAC cells through iodoTMT-based quantitative proteomics, which identified over 4300 oxidized cysteine sites in more than 2100 proteins in HPDE6c7 and PANC-1 cells. Among them, 1715 cysteine residues were shown to be differentially oxidized between HPDE6c7 and PANC-1 cells. Also, charged amino acids including aspartate, glutamate and lysine were significantly overrepresented in flanking sequences of oxidized cysteines. Differentially oxidized proteins in PANC-1 cells were enriched in multiple cancer-related biological processes and signaling pathways. Specifically, the HIF-1 signaling proteins exhibited significant oxidation alterations in PANC-1 cells, and the reduced PHD2 oxidation in human PDAC tissues was correlated with lower survival time in pancreatic cancer patients.ConclusionThese investigations provided new insights into protein oxidation-regulated signaling and biological processes during PDAC pathogenesis, which might be further explored for pancreatic cancer diagnosis and treatment."

基金机构：National Natural Science Foundation of China

基金资助正文：Not applicable.