CD36-mediated podocyte lipotoxicity promotes foot process effacement

作者全名："Hua, Wei; Peng, Lan; Chen, Xue-mei; Jiang, XuShun; Hu, JianGuo; Jiang, Xian-Hong; Xiang, Xu; Wan, Jiangmin; Long, Yingfei; Xiong, Jianqiong; Ma, Xueyi; Du, Xiaogang"

作者地址："[Du, Xiaogang] Chongqing Med Univ, Affiliated Hosp 1, Dept Nephrol, Youyi Rd 1, Chongqing 400042, Peoples R China; [Hua, Wei; Jiang, Xian-Hong; Xiang, Xu; Ma, Xueyi] Chongqing Med & Pharmaceut Coll, Affiliated Hosp 1, Dept Nephrol, Chongqing 400000, Peoples R China; [Peng, Lan] Chongqing Med & Pharmaceut Coll, Basic Dept, Chongqing 401331, Peoples R China; [Chen, Xue-mei] Chongqing Med Univ, Affiliated Hosp 1, Emergency Dept, Chongqing 400042, Peoples R China; [Jiang, XuShun] Chongqing Med Univ, Affiliated Hosp 1, Dept Nephrol, Chongqing 400042, Peoples R China; [Hu, JianGuo] Chongqing Med Univ, Affiliated Hosp 2, Dept Obstet & Gynecol, Chongqing 401420, Peoples R China; [Wan, Jiangmin] Peoples Hosp Qijiang Dist, Dept Nephrol, Chongqing 401420, Peoples R China; [Long, Yingfei; Xiong, Jianqiong] Chongqing Med Univ, Affiliated Hosp 3, Dept Obstet & Gynecol, Chongqing 401120, Peoples R China; [Du, Xiaogang] Hosp Chongqing Univ, Chongqing 400044, Peoples R China"

通信作者："Du, XG (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Nephrol, Youyi Rd 1, Chongqing 400042, Peoples R China."

来源：OPEN MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001187148700001

JCR分区：Q2

影响因子：1.7

年份：2024

卷号：19

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：CD36; podocyte; foot process effacement; palmitic acid; ROS

摘要："Background Lipid metabolism disorders lead to lipotoxicity. The hyperlipidemia-induced early stage of renal injury mainly manifests as podocyte damage. CD36 mediates fatty acid uptake and the subsequent accumulation of toxic lipid metabolites, resulting in podocyte lipotoxicity. Methods Male Sprague-Dawley rats were divided into two groups: the normal control group and the high-fat diet group (HFD). Podocytes were cultured and treated with palmitic acid (PA) and sulfo-N-succinimidyl oleate (SSO). Protein expression was measured by immunofluorescence and western blot analysis. Boron-dipyrromethene staining and Oil Red O staining was used to analyze fatty acid accumulation. Results Podocyte foot process (FP) effacement and marked proteinuria occurred in the HFD group. CD36 protein expression was upregulated in the HFD group and in PA-treated podocytes. PA-treated podocytes showed increased fatty acid accumulation, reactive oxygen species (ROS) production, and actin cytoskeleton rearrangement. However, pretreatment with the CD36 inhibitor SSO decreased lipid accumulation and ROS production and alleviated actin cytoskeleton rearrangement in podocytes. The antioxidant N-acetylcysteine suppressed PA-induced podocyte FP effacement and ROS generation. Conclusions CD36 participated in fatty acid-induced FP effacement in podocytes via oxidative stress, and CD36 inhibitors may be helpful for early treatment of kidney injury."

基金机构：First Affiliated Hospital of Chongqing Medical and Pharmaceutical College [201603]; First Affiliated Hospital of Chongqing Medical and Pharmaceutical College [201701]; Nan'an District Health Commission of Chongqing [2017-19]

基金资助正文："This work was supported by the The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College under Grant No. 201603, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College under Grant No. 201701, and Nan'an District Health Commission of Chongqing under Grant No. 2017-19."