Construction of an acellular spinal cord scaffold with a novel NT-3 sustained-release system promotes the neural differentiation of BMSCs through the NT-3/TrkC pathway

作者全名："Jiang, Tao; Yin, Hong; Yu, Miao; Wang, Han; Xing, Hui"

作者地址："[Jiang, Tao; Yin, Hong; Yu, Miao] Army Med Univ, Xinqiao Hosp, Dept Orthoped, Chongqing 400037, Peoples R China; [Wang, Han] Chongqing Med Univ, Affiliated Hosp 1, Dept Orthoped, Chongqing 400016, Peoples R China; [Xing, Hui] Chongqing Med Univ, Affiliated Hosp 3, Dept Ctr Orthoped & Trauma, Chongqing 401120, Peoples R China"

通信作者："Xing, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Dept Ctr Orthoped & Trauma, Chongqing 401120, Peoples R China."

来源：AIP ADVANCES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001187339800007

JCR分区：Q4

影响因子：1.4

年份：2024

卷号：14

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："In previous neural tissue engineering studies, we successfully constructed NT-3 cross-linked acellular spinal cord scaffolds (NT-3 cross-linked scaffolds), which can sustain the release of NT-3 and promote the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells. However, the molecular mechanism by which NT-3 cross-linked scaffolds promote BMSC differentiation into neurons is unknown, coupled with the low drug loading of scaffolds and the sudden release of NT-3 on the first day. We used WB and PCR in combination with NT-3/TrkC, MAPK/ERK, and PI3K/Akt pathway inhibitors to determine the mechanism of action in vitro. We hypothesized that NT-3 mediates the NT-3/TrkC pathway as a major target molecule that promotes the differentiation of BMSCs into neurons. We prepared an improved NT-3 scaffold and improve the sustained release of NT-3 through the combination of heparin methacryloyl and EDC/NHS. The adhesion, proliferation, differentiation, and NT-3/TrkC signaling pathway of BMSCs on different scaffolds were analyzed. We concluded that NT-3-improved scaffolds can be loaded with more NT-3 and more effectively promote the differentiation of BMSCs into neurons through the NT-3/TrkC pathway. The proposed method has biocompatibility and provides a new idea for spinal cord repair."

基金机构："Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commissionhttps://doi.org/10.13039/501100012669 [cstc2020jcyj-msxmX0278]; Natural Science Foundation of Chongqing [2021((sic))26]; Science and Technology Planning project contract of Yubei District, Chongqing (Application technology research-invention and achievement transformation special project)"

基金资助正文："This research was sponsored by Natural Science Foundation of Chongqing (Grant No. cstc2020jcyj-msxmX0278). It was also sponsored by the Science and Technology Planning project contract of Yubei District, Chongqing (Application technology research-invention and achievement transformation special project), Contract number: 2021((sic))26. We are grateful to the staff at Xinqiao Hospital for providing the animal's tissues for our experiment."