ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells

作者全名："Lin, Ting; Zhang, Shuxian; Tang, Yi; Xiao, Ming; Li, Ming; Gong, Hanjuan; Xie, Hailun; Wang, Yalan"

作者地址："[Lin, Ting; Zhang, Shuxian; Tang, Yi; Xiao, Ming; Li, Ming; Gong, Hanjuan; Xie, Hailun; Wang, Yalan] Chongqing Med Univ, Basic Med Coll, Mol Med & Canc Res Ctr, Dept Pathol, Chongqing 400016, Peoples R China; [Zhang, Shuxian; Xiao, Ming; Wang, Yalan] Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing 400016, Peoples R China; [Tang, Yi; Li, Ming; Wang, Yalan] Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing 400016, Peoples R China"

通信作者："Wang, YL (通讯作者)，Chongqing Med Univ, Basic Med Coll, Mol Med & Canc Res Ctr, Dept Pathol, Chongqing 400016, Peoples R China.; Wang, YL (通讯作者)，Chongqing Med Univ, Mol Med Diagnost & Testing Ctr, Chongqing 400016, Peoples R China.; Wang, YL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Pathol, Chongqing 400016, Peoples R China."

来源：BMC CANCER

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001187772200008

JCR分区：Q2

影响因子：3.4

年份：2024

卷号：24

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：ART1; Cell proliferation; Colorectal cancer; gp130; IL-6

摘要："Colorectal cancer (CRC) is a worldwide health concern. Chronic inflammation is a risk factor for CRC, and interleukin-6 (IL-6) plays a pivotal role in this process. Arginine-specific mono-ADP-ribosyltransferase-1 (ART1) positively regulates inflammatory cytokines. ART1 knockdown reduces the level of glycoprotein 130 (gp130), a key transducer in the IL-6 signalling pathway. However, the relationship between ART1 and IL-6 and the resulting effects on IL-6-induced proliferation in CRC cells remain unclear. The aims of this study were to investigate the effects of ART1 knockdown on IL-6-induced cell proliferation in vitro and use an in vivo murine model to observe the growth of transplanted tumours. The results showed that compared with the control, ART1-sh cancer cells induced by IL-6 exhibited reduced viability, a lower rate of colony formation, less DNA synthesis, decreased protein levels of gp130, c-Myc, cyclin D1, Bcl-xL, and a reduced p-STAT3/STAT3 ratio (P < 0.05). Moreover, mice transplanted with ART1-sh CT26 cells that had high levels of IL-6 displayed tumours with smaller volumes (P < 0.05). ART1 and gp130 were colocalized in CT26, LoVo and HCT116 cells, and their expression was positively correlated in human CRC tissues. Overall, ART1 may serve as a promising regulatory factor for IL-6 signalling and a potential therapeutic target for human CRC."

基金机构：Innovation Project of Graduate Student in Chongqing

基金资助正文："The authors thank Professor Yu Quan Wei (Sichuan University, Chengdu, China), Professor Wei Xue Tang (Chongqing Medical University, Chongqing, China) and Professor Zhi Dong (Chongqing Medical University, Chongqing, China) for providing cell lines."