Construction of programmed time-released multifunctional hydrogel with antibacterial and anti-inflammatory properties for impaired wound healing

作者全名："Peng, Yuan; Guo, Yicheng; Ge, Xin; Gong, Yali; Wang, Yuhan; Ou, Zelin; Luo, Gaoxing; Zhan, Rixing; Zhang, Yixin"

作者地址："[Peng, Yuan; Zhang, Yixin] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Plast & Reconstruct Surg, 639 Zhi Zao Ju Rd, Shanghai 200011, Peoples R China; [Guo, Yicheng; Gong, Yali; Wang, Yuhan; Ou, Zelin; Luo, Gaoxing; Zhan, Rixing] Army Med Univ, Mil Med Univ 3, State Key Lab Trauma & Chem Poisoning, Inst Burn Res,Southwest Hosp, Chongqing 400038, Peoples R China; [Ge, Xin] Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, Chongqing 400010, Peoples R China"

通信作者："Zhang, YX (通讯作者)，Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Plast & Reconstruct Surg, 639 Zhi Zao Ju Rd, Shanghai 200011, Peoples R China.; Luo, GX; Zhan, RX (通讯作者)，Army Med Univ, Mil Med Univ 3, State Key Lab Trauma & Chem Poisoning, Inst Burn Res,Southwest Hosp, Chongqing 400038, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001189272600003

JCR分区：Q1

影响因子：10.6

年份：2024

卷号：22

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Nanozymes; Time-released hydrogel; Antibacterial; Anti-inflammation; Wound healing

摘要："The successful reprogramming of impaired wound healing presents ongoing challenges due to the impaired tissue microenvironment caused by severe bacterial infection, excessive oxidative stress, as well as the inappropriate dosage timing during different stages of the healing process. Herein, a dual-layer hydrogel with sodium alginate (SA)-loaded zinc oxide (ZnO) nanoparticles and poly(N-isopropylacrylamide) (PNIPAM)-loaded Cu5.4O ultrasmall nanozymes (named programmed time-released multifunctional hydrogel, PTMH) was designed to dynamically regulate the wound inflammatory microenvironment based on different phases of wound repairing. PTMH combated bacteria at the early phase of infection by generating reactive oxygen species through ZnO under visible-light irradiation with gradual degradation of the lower layer. Subsequently, when the upper layer was in direct contact with the wound tissue, Cu5.4O ultrasmall nanozymes were released to scavenge excessive reactive oxygen species. This neutralized a range of inflammatory factors and facilitated the transition from the inflammatory phase to the proliferative phase. Furthermore, the utilization of Cu5.4O ultrasmall nanozymes enhanced angiogenesis, thereby facilitating the delivery of oxygen and nutrients to the impaired tissue. Our experimental findings indicate that PTMHs promote the healing process of diabetic wounds with bacterial infection in mice, exhibiting notable antibacterial and anti-inflammatory properties over a specific period of time."

基金机构："State Key Laboratory of Trauma, Burns and Combined Injury [82372514]; National Natural Science Foundation of China [SKLKF202208]; State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University [18XD1423700]; Outstanding Professional and Technical Leader Program of the Shanghai Municipal Science and Technology Commission [20152227]; Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support"

基金资助正文："Y.P., Y.C.G. and X.G. contributed equally to this work. This work was supported by the National Natural Science Foundation of China (Grant No. 82372514), the State Key Laboratory of Trauma and Chemical Poisoning, Army Medical University (Grant No. SKLKF202208), the Outstanding Professional and Technical Leader Program of the Shanghai Municipal Science and Technology Commission (18XD1423700) and Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (Grant number: 20152227)."