"Label-Free, Sensitive, and Versatile Colorimetric Method for Molecule Detection via the G-Quadruplex-Based Signal Quenching Strategy"

作者全名："Mei, Qiang; Gu, Baiwen; Jiang, Yinyu; Wang, Yulin; Lai, Weiju; Chen, Hu; Chen, Jide; Zhao, Xianxian"

作者地址："[Mei, Qiang; Jiang, Yinyu; Wang, Yulin] Chongqing Pharmaceut Exchange Co Ltd, Equipment Trading Div, Chongqing 401336, Peoples R China; [Gu, Baiwen; Lai, Weiju; Chen, Hu; Zhao, Xianxian] Chongqing Univ, Cent Lab, FuLing Hosp, Chongqing 408099, Peoples R China; [Chen, Jide] Chongqing Med Univ, Dept Urol, Bishan Hosp, Chongqing 402760, Peoples R China"

通信作者："Zhao, XX (通讯作者)，Chongqing Univ, Cent Lab, FuLing Hosp, Chongqing 408099, Peoples R China.; Chen, JD (通讯作者)，Chongqing Med Univ, Dept Urol, Bishan Hosp, Chongqing 402760, Peoples R China."

来源：ACS OMEGA

ESI学科分类：CHEMISTRY

WOS号：WOS:001189802800001

JCR分区：Q2

影响因子：3.7

年份：2024

卷号：9

期号：13

开始页：15350

结束页：15356

文献类型：Article

关键词：　

摘要："Signal amplification strategies have emerged as a prominent tool in the field of improving the detection sensitivity of small extracellular vesicles (sEVs). It is important to highlight that the utilization of signal quenching strategies is not commonly implemented. A detection technique for sEVs was established based on the unwinding of G-quadruplex using Klenow fragment polymerase (KF), which served as an inspiration for this study. This system is characterized by its simplicity and lack of labeling, making it an efficient approach for signal quenching. In the presence of sEVs, the CD63 aptamer in the capture@sMBs complex binds with the CD63 protein on the surface of sEVs to release trigger sequences, which were employed as a primer to mediate the DNA polymerase/endonuclease-assisted signal recycling. The signal recycling process produces numerous single-stranded DNA sequences that can bind to the toehold section of the G-quadruplex. This leads to the rupture of the G-quadruplex structure and the subsequent deactivation of a DNAzyme generated by the G-quadruplex structure and hemin, thereby inhibiting its biological catalytic function. Consequently, the G-quadruplex structure would undergo a transformation to a duplex structure, leading to the emergence of a discernible differential signal that can be noticed in a majority of instances, even without the aid of magnification devices. The decrease in the prominent signal allows for the efficient analysis of target sEVs, which exhibit a notably low detection limit. In addition to the detection of sEVs, the approach has also been utilized for the investigation of miRNA-21. The approach demonstrates a high level of selectivity and robustness in its capacity to differentiate between target miRNA and base-mismatched miRNA as well as other miRNA families. This statement suggests that the assay holds significant promise for use in biochemical research and clinical diagnosis."

基金机构：National Natural Science Foundation of China [82202645]; National Natural Science Foundation of China

基金资助正文：The authors thank the financial support from the National Natural Science Foundation of China (no. 82202645);